Development of Stability-Indicating Liquid Chromatographic Method for Analysis of Cefoxitin Sodium in Sterile Formulation.
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2012, Vol 1, Issue 2
Abstract
Stability-indicating high performance liquid chromatography (HPLC) method was developed and validated for analysis of cefoxitin sodium in its powder for injection dosage form. Chromatographic separation was achieved on a C18 µBondapack (300×3.9, 10µ) column, maintained at 30°C with a mobile phase consisted of water: acetonitrile: glacial acetic acid (800:190:10) and a flow rate of 0.9mL/min. The peak was detected at 254nm & the retention time was obtained at 16.74 min. The peak area plot was linear over the concentration range of 72.16µg/mL to 451.04µg/mL. The different experimental parameters affecting the drug stability were optimized. The method was validated for accuracy, precision, reproducibility, specificity, robustness and ruggedness in accordance with International Conference on Harmonization (ICH) guidelines. The proposed method was successfully applied for the analysis of cefoxitin sodium in drug substance and drug product in the presence of hydrolytic and oxidative degradants.
Authors and Affiliations
R. Revathi*| Dept of Pharmaceutical Analysis, The Erode College of Pharmacy, Erode - 638112, Tamil Nadu,, N. Reddiyar Lisha| Dept of Pharmaceutical Analysis, The Erode College of Pharmacy, Erode - 638112, Tamil Nadu,, Sanjay Dhumal| Hospira India Pvt LTD, Chennai..
Keywords
Related Articles
- EP ID EP1509
- DOI -
- Views 642
- Downloads 37
BIOLOGICALLY ACTIVE SECONDARY METABOLITES FROM KALANCHOE TOMENTOSA
Phytochemical study of the ethanolic extract of Kalanchoe tomentosa (Crassulaceae) resulted in the isolation of 14 compounds identified as; α-amyrin acetate (1), friedelin (2), glutinol (3), 1-dotriacontanol (4), phytol...
SOLUBLITY ENHANCEMENT OF FLUCONAZOLE BY FORMULATION OF HYDROTROPIC SOLID DISPERSIONS
In the present research work mixed hydrotropic solubilization phenomenon is used to enhance the solubilization of poorly water soluble drugs. Bulk drug samples were identified by the observed IR spectra and the melting p...
ANTIOXIDANT AND ANTIDIABETIC ROLE OF PETROSELINUM CRISPUM AGAINST STZ-INDUCED DIABETES IN RATS
Petroselinum crispum (Parsley) has been reported to have a number of possible medicinal attributes. The aim of this work is to evaluate the ameliorative effect of parsley aqueous extract (PAE) against STZ-induced diabeti...
RECTAL MISOPROST VS INTA MUSCULAR SYNTOCINON IN THE MANAGEMENT OF THIRD STAGE OF LABOUR
CHARACTERISATION AND OPTIMISATION OF BIOSURFACTANT PRODUCED BY PSEUDOMONAS FLUORESCENS FROM OIL CONTAMINATED SOIL
The biosurfactant producing strain Pseudomonas fluorescens was isolated from Dharmapuri District, Tamil Nadu. India. The biosurfactant produced by Pseudomonas fluorescens was able to reduce the surface tension of media...