Development of THP assay and clinical applications
Journal Title: Journal of Analytical Bio-Science - Year 2011, Vol 34, Issue 2
Abstract
Tamm-Horsfall protein (uromodulin) is the most abundant glycoprotein in human urine. It is synthesized exclusively in the thick ascending limb of the loop of Henle. Its biological function remains unclear. Qualitative and quantitative analyse have generally been done using been SDS-polyacrylamide gel electrophoresis and enzyme-linked immunosorbent assay (ELISA) respectively. Our established ELISA yielded a linearity within a range of 1.25 ng/ml to 80 ng/ml and a sensitivity of 0.78 ng/ml. It has recently been demonstrated that familial juvenile hyperuricemic nephropathy and medullary cystic kidney disease are caused by THP mutations. Some researchers have developed a non-invasive quantitative assay for urinary THP as a potential biomarker for the diagnosis of a kidney stone disease. In this article, we review previous quantitative analyse of THP and their clinical relevance in THP expression and excretion.
Authors and Affiliations
Hiroji Shimomura
Keywords
Related Articles
- EP ID EP97300
- DOI -
- Views 152
- Downloads 0
Monomeric sarcosine oxidase acts on both L- and D-substrates
The stereoselectivity of monomeric sarcosine oxidase (mSox), an enzyme used for creatinine determination, was assayed by using N-methylalanine and proline. As a result, mSox reacted to both L- and D-substrates, although...
Blood clotting time tests using an automated coagulation analyzer−ACL TOP series, STACIA−
The hemostatic mechanism is very important for achieving a vital self-defense. The blood-clotting-time is the test to examine coagulation reaction, including both the extrinsic and intrinsic coagulation systems, whether...
Dimension system for disaster control
Disasters occur due to a variety of factors such as human or natural causes. Japan has especially been damaged by earthquakes from past to present. It is also clear that there are great possibilities that Japan will be e...
Reference range study in a neuron-specific enolase (NSE) assay
Neuron-specific enolase (NSE) is widely used as a marker of small-cell lung cancer. Upper reference range for total plasma NSE level in adults is usually 12.0 ng/mL, as arranged by the manufacturer of the measurement kit...
Prevention of lifestyle-related diseases in school children
Lifestyle-related diseases, including dyslipidaemia, hypertension, hyperinsulinaemia, and obesity, are recognized as major risk factors for cardiovascular disease in adults. There is convincing evidence that lifestyle-re...