Development of THP assay and clinical applications
Journal Title: Journal of Analytical Bio-Science - Year 2011, Vol 34, Issue 2
Abstract
Tamm-Horsfall protein (uromodulin) is the most abundant glycoprotein in human urine. It is synthesized exclusively in the thick ascending limb of the loop of Henle. Its biological function remains unclear. Qualitative and quantitative analyse have generally been done using been SDS-polyacrylamide gel electrophoresis and enzyme-linked immunosorbent assay (ELISA) respectively. Our established ELISA yielded a linearity within a range of 1.25 ng/ml to 80 ng/ml and a sensitivity of 0.78 ng/ml. It has recently been demonstrated that familial juvenile hyperuricemic nephropathy and medullary cystic kidney disease are caused by THP mutations. Some researchers have developed a non-invasive quantitative assay for urinary THP as a potential biomarker for the diagnosis of a kidney stone disease. In this article, we review previous quantitative analyse of THP and their clinical relevance in THP expression and excretion.
Authors and Affiliations
Hiroji Shimomura
Keywords
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