Monomeric sarcosine oxidase acts on both L- and D-substrates
Journal Title: Journal of Analytical Bio-Science - Year 2012, Vol 35, Issue 5
Abstract
The stereoselectivity of monomeric sarcosine oxidase (mSox), an enzyme used for creatinine determination, was assayed by using N-methylalanine and proline. As a result, mSox reacted to both L- and D-substrates, although heterotetrameric sarcosine oxidase has already been known as an L-specific enzyme. The catalytic efficiency of mSox for N-methyl-L-alanine was higher than that for N-methyl-D-alanine, while the efficiency for L-proline was lower than that for D-proline. Those substrate specificities were discussed in terms of the enzyme-substrate docking models constructed.
Authors and Affiliations
Yoshiaki Nishiya
Keywords
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