High sensitive determination of oxysterols in human plasma using gas chromatography-mass spectrometry (GC-MS)
Journal Title: Journal of Analytical Bio-Science - Year 2012, Vol 35, Issue 2
Abstract
Oxysterols, cholesterol oxidation products, have been important markers for various diseases. For example, it is suggested that 24S-hydroxycholesterol, 27-hydroxycholesterol and 7-ketocholesterol can be used as a marker of Alzheimer's disease, cerebrotendinous xanthomatosis and diabetes mellitus, respectively. Therefore, it is necessary to determine the concentration levels of individual oxysterols in plasma. In this report, we have described a pretreatment procedure of human plasma for the simultaneous determination of oxysterols using gas chromatography-mass spectrometry (GC-MS). Pretreatments were carried out as follows; extraction from lipoprotein, saponification by hydrolyzing oxysterols esters, extraction with n-hexane, solid-phase extraction to remove the large levels of cholesterol in plasma, elution of oxysterols from their solid-phase, and their derivatization by trimethylsilyl reagent. Twelve oxysterols were simultaneously determined using this pretreatment procedure and a GC-MS analysis. The procedure is a simple and useful one for application to human plasma.
Authors and Affiliations
Saori Nakagawa, Susumu Yamato
Keywords
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