DEVELOPMENT OF UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF COLCHICINE IN PHOSPHATE BUFFER SALINE pH 6.4
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 2
Abstract
The present investigation explores a method to analyze colchicine in phosphate buffer saline pH 6.4. Based on the spectrophotometric characteristics of colchicine, λmax selected was 353.8 nm. Analytical parameters validated were linearity, accuracy, precision, LOD, LOQ and robustness. The developed method demonstrated exemplary linearity in phosphate buffer saline pH6.4 (R2 = 0.995) for concentration of 6-22 µg/ml with linear equation of y=0.037x+0.005. LOD and LOQ obtained were found to be i.e. 0.145µg/ml and 0.483µg/ml respectively. All validation parameters were observed to be within acceptable range, as recommended by ICH guidelines. The above results confirmed the validity of proposed method for routine estimation of colchicine in phosphate buffer saline pH6.4.
Authors and Affiliations
Arpna patial, Purnima verma
Keywords
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