DEVELOPMENT OF UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF COLCHICINE IN PHOSPHATE BUFFER SALINE pH 6.4
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 2
Abstract
The present investigation explores a method to analyze colchicine in phosphate buffer saline pH 6.4. Based on the spectrophotometric characteristics of colchicine, λmax selected was 353.8 nm. Analytical parameters validated were linearity, accuracy, precision, LOD, LOQ and robustness. The developed method demonstrated exemplary linearity in phosphate buffer saline pH6.4 (R2 = 0.995) for concentration of 6-22 µg/ml with linear equation of y=0.037x+0.005. LOD and LOQ obtained were found to be i.e. 0.145µg/ml and 0.483µg/ml respectively. All validation parameters were observed to be within acceptable range, as recommended by ICH guidelines. The above results confirmed the validity of proposed method for routine estimation of colchicine in phosphate buffer saline pH6.4.
Authors and Affiliations
Arpna patial, Purnima verma
Keywords
Related Articles
- EP ID EP103327
- DOI -
- Views 84
- Downloads 0
COMPARISON OF FREE RADICAL SCAVENGING ACTIVITY OF TWO MAIN VARIETIES OF CICER ARIETINUM SPROUTS
The purpose of the study was to evaluate the extent of free radical scavenging properties and antioxidant effects of crude extracts of sprouted Cicer arietinum (Chick pea/Chana/Bengal gram) seeds. Two main varieties of C...
HEAVY METALS IN ENVIRONMENT, LIVING SYSTEMS AND HERBAL PREPARATION: AN OVERVIEW
Heavy metals occur as a natural constituent of earth’s crust. Due to the indiscriminate human activities there is drastic change in geochemical and biochemical balance which results in accumulation of metals in plant par...
CLINICAL EVALUATION OF DHANVANTARI TAILA IN THE MANAGEMENT OF INFERTILITY WITH DYSFUNCTIONAL UTERINE BLEEDING
This is a clinical study of dysfunctional uterine bleeding in infertile patients. After evaluating the cause for infertility is dysfunctional Uterine bleeding I choose treatment with Dhanvantari Taila as Uttara Vasti int...
VALIDATED REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS ESTIMATION OF OLMESARTAN MEDOXOMIL, HYDROCHLOROTHIAZIDE AND AMLODIPINE BESYLATE IN NEWLY DESIGNED PHARMACEUTICAL DOSAGE FORM
A Simple, accurate, and precise reverse phase high performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of Olmesartan Medoxomil, Hydrochlorothiazide and Amlodipine Besylate in marketed formu...
EVALUATION OF ANXIOLYTIC ACTIVITY OF BOERHAAVIA DIFFUSA HYDRO-ALCOHOLIC EXTRACT OF LEAVES IN RATS
The hydro-alcoholic extract of Boerhaavia diffusa of leaves was investigated for evaluation of exhibited anxiolytic activity in rats at a dose 100 and 200 mg/kg by p.o. route for Elevated Plus Maze test, Hole Board Test...