DEVELOPMENT, STANDARDIZATION AND VALIDATION OF HPLC METHOD FOR ESTIMATION OF βSITOSTEROL IN VITEX NEGUNDO SEED EXTRACT
Journal Title: Int J of Pharm Rev& Res - Year 2015, Vol 5, Issue 2
Abstract
A simple, precise and accurate high-performance liquid chromatographic method has been developed for determination of β-sitosterol in the hydroalcoholic extact of Vitex negundo Linn. A Reverse phase HPLC column CrestPack RP C18 (Make: Jasco; 250 mm x 4.6 mm I.D; particle size 5μm) was used as stationary phase with a mobile phase comprising of acetonitrile : methanol (70:30) v/v at a flow rate of 1.0 mL min-1 and UV detection at 210nm with a run time of 6 mins. The proposed method was validated for linearity, accuracy, precision, LOD and LOQ. LOD for beta-sitosterol was found to be 0.5μg/mL whereas LOQ for the same was found to be 1.5μg/mL. The method was found to be linear over the range 1 μg/mL to 5μg/mL with coefficient of regression 0.9732 for betasitosterol. Intra-day and inter-day precision studies showed % RSD to be less than 2 and hence the method was precise. Stock solution stability studies were carried out for beta-sitosterol. Stability samples in methanol after a week revealed that all beta-sitosterol in solution form were stable. The validated HPLC method can be used for a routine quality control analysis and quantitation of β-sitosterol from Vitex negundo Linn.
Authors and Affiliations
Divya K Shetty , Swati S Patil
Keywords
Related Articles
- EP ID EP106564
- DOI -
- Views 166
- Downloads 0
CHRONOMODULATED DRUG DELIVERY SYSTEM: A REVIEW
In this review, the concepts of biological rhythms, chronobiology, chronopharmacology, and chronotherapy for various diseases have been discussed. Chronopharmaceutical Drug Delivery Systems (ChrDDS) is novel system which...
A REVIEW ON LIQUISOLID TECHNOLOGY
The “Liquisolid” technique is a novel and capable addition towards such an aims for solubility enhancement and dissolution improvement, thereby it increases the bioavailability. This technique is based upon the admixture...
A REVIEW ON PENICILLIN ALLERGIES
Penicillin allergies are the most common miss diagnosed and wrongly diagnosed allergies in children. Many children are labeled penicillin allergic without getting them tested prior. Misdiagnosis of penicillin allergy may...
TRADITIONAL MEDICINAL HERBS FOR ASTHMA: A CRITICAL REVIEW
This review describes information on the efficacy of Ayurvedic herbs and some plant originated medicine in bronchial asthma (Shwasa roga). It as a substance or product that is used or intended to be used, to modify or ex...
PROMISING PHARMACEUTICAL PROSPECTIVE OF ‘JAVA OLIVE’- STERCULIA FOETIDA LINN (STERCULIACEAE)
Medicinal trees have been used both in the prevention and cure of various diseases of human and their pets with the advent of human civilization. Many system of therapy have been developed primarily based on plant. Plant...