Diagnosis of Genotoxicity by Comet Assay in Oral Cancer of Puducherry Population.
Journal Title: International Journal of Medical Science and Innovative Research (IJMSIR) - Year 2018, Vol 3, Issue 12
Abstract
Background: Single cell gel electrophoresis (SCGE) or “comet assay” is a simple, sensitive and non-invasive fluorescent microscopic method for measuring and analyzing single stranded DNA breaks at individual cell level. Oxidative stress and poorly maintained antioxidant defense system may leads to increased intracellular reactive oxygen species (ROS) that can control basic cellular functions, such as proliferation and apoptosis which leads to the development of cancer. Aim and Objectives: To evaluate total antioxidant status (TAS) and the extent of oxidative stress (OS) by measuring DNA damage in oral leukoplakia and oral squamous cell carcinoma (OSCC) in comparison to normal healthy individuals. Methods: A total of 90 subjects aged 30 – 70 were included for the study. Of these 30 were healthy controls, 25 patients with oral leukoplakia and 35 clinically and histologically diagnosed patients with OSCC. Blood samples were evaluated for malondialdehyde (MDA), comet assay and antioxidants. All study variables assessed through Kolmogorov-Smirnov one sample test (K-S test). Comparison among multiple groups was performed by one-way ANOVA with LSD post hoc test. Correlation of DNA damage with TAS was assessed by Pearson correlation coefficient. Conclusions: Significant increase in DNA damage with decreased antioxidants status was observed in oral leukoplakia compared to OSCC patients and controls. TAS and lymphocyte DNA damage showed a strong negative correlation among three groups. Oral cancer associated with OS causes genotoxic susceptibility in cancer. Lack of DNA repair mechanisms causes extensive DNA damage in oral leukoplakia patients, suggesting that OS is important in the pathogenesis of oral cancer.
Authors and Affiliations
Dr. Subash P,
Keywords
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