Diagnosis of Urological Cancer by 1H NMR Based Metabonomics Urinalysis: A Pilot Study
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2017, Vol 19, Issue 3
Abstract
Aims: The most prevalent urological malignancies are prostate cancer (PC), bladder cancer (BC) and renal cancer (RC). The diagnosis of each of these diseases is conducted, in most cases, invasively and each procedure may lead to complications. The method of metabonomic spectrometry by nuclear magnetic resonance of hydrogen (1H NMR) provides pathways of diagnostic information that can identify pathologies without invasive procedures. The possibility of using this method for the diagnosis of those cancers by a single sample of urine has not been described yet. Study Design: Prospective, observational. Place and Duration of the Study: Department of Urology and Department Fundamental Chemistry of Universidade Federal de Pernambuco (UFPE), between July of 2015 to February of 2016. Methodology: A sample of 3 ml of urine was collected from 25 volunteers distributed into 4 groups: A control group (07 volunteers), a PC (08 volunteers), a BC (05 volunteers), and an RC (05 volunteers). All samples underwent 1H MRI to generate spectra. A multivariate statistics analysis for the development of metabonomic models and comparison analysis groups was performed. Results: These models showed a slight separation between the control group and each of the three groups of patients with oncological diseases. For the elaboration of the definitive models it was necessary to incorporate the volunteers of the BC and RC into one group (BC/RC). The metabonomic method when compared to control group, shown sensitivity of 90.9%, specificity of 100%, 100% PPV and NPV of 85.7% for CB/CR and sensitivity, specificity, PPV and NPV of 100% for the PC. Conclusion: This pilot study demonstrates that the method is feasible with easy execution, showing simplicity besides being not invasive and allowing the diagnosis of oncological diseases with a single urine collection.
Authors and Affiliations
Leslie Clifford Noronha Araújo, Flávia Cristina Morone Pinto, Tássia Brena Barroso Carneiro Costa, Ronaldo Dionísio Silva, Salvador Vilar Correia Lima, Ricardo Oliveira Silva
Keywords
Related Articles
- EP ID EP311090
- DOI 10.9734/BJMMR/2017/30340
- Views 67
- Downloads 0
Patients’ Information Leaflets: Its’ Influence on Ophthalmic Patient Education and Medication Compliance
Aims: To investigate the influence of patient information leaflets on Ophthalmic patients’ education and medication compliance. Study Design: Hospital-based cross- sectional survey. Place and Duration of Study: Departmen...
Post Surgery Treatment with Topical Eye Drops of the Recurrent Conjunctival Melanoma
Conjunctival malignant melanoma (CMM) is rare and can be life threatening. Management of CMM is by surgical excision of the tumor. Non-surgical treatment consists of topical eye drops especially in diffuse and multifocal...
Study on Self-medication Practices among University Students of Bangladesh
Aims: To know how much knowledge and awareness university students of Bangladesh have about commonly used medicines. Study Design: The study was conducted on 1200 students randomly selected from 9 university of Banglades...
Chronic Recurrent Hematogenous Osteomyelitis (Literature Review)
The analyses of the existed literature devoted to chronic recurrent hematogenous osteomyelitis have been performed in this study. Pathogenesis and morphologic picture of the disease have been studied in detail. It has be...
Calcium Polycarbophil in the Management of Fecal Incontinence
Background: Few clinical studies have assessed the efficacy of calcium polycarbophil that is currently recommended as an ideal and initial bulk-forming agent in patients with fecal incontinence. Aims: The aim of this st...