Diagnostic Efficacy of Different Techniques for Detection of Helicobacter. Pylori in Upper Gastrointestinal Tract Pathologies
Journal Title: International Journal of Contemporary Research and Review - Year 2017, Vol 8, Issue 6
Abstract
Aim:Helicobacter pyloriinfection is very common worldwide and causes a wide spectrum of diseases. A reliable diagnosis is crucial for patients withH. pylori-related diseases. Hence comes the role of detection of H.pylori using various invasive and noninvasive methods.Material and Methods:We conducted a study on 176 patients suffering from various gastrointestinal complaints who were screened forH. pyloriinfection using invasive tests like rapid urease test (RUT) on fresh biopsy and histological examination using H & E, Giemsa and polyclonal H.pylori antibody.Results:Results werederived in terms of sensitivity and specificity with each test and compared. Rapid urease test gave positivity in 40 cases with Sensitivityand Specificity of 87% and 100% respectively while H& E showed sensitivity of 96% and specificity of 98%.Giemsa was positive in maximum number of cases with no false negative hence Sensitivity and Specificity calculated is 100% and 98% respectively. Immunohistochemistry with polyclonal H.pylori antibody gave no false positive or false negative result leading tohighestrate of sensitivity and specificity of 100%each.Conclusion:Our studyconcluded that Giemsa and Polyclonal H.pylori antibodybothhave a very high sensitivity in detecting H.pylori. While giemsa is inexpensive, less time consuming and is sufficient for routine diagnosis of the bacteria the latter has maximum specificity and differentiates H.pylori from its other morphological mimickers
Authors and Affiliations
A. R. Piyush,Roobina Khan, Hasan Harris,Veena Maheshwari
Keywords
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- EP ID EP314257
- DOI 10.15520/ijcrr/2017/8/06/201
- Views 86
- Downloads 0
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