Diagnostic Perfofmance of Ziehl-Neelsen, Flourescent Auramine O Stains and Genexpert for the Detection of Mycobacterium Tuberculosis
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 17, Issue 2
Abstract
Tuberculosis (TB), one of the oldest known diseases and still a major cause of mortality today, many other organ systems such as the bone and central nervous system are manifested and affected by the disease. It is caused by a closely-related group of organisms, all of which form the Mycobacterium tuberculosis complex. These organisms include M. tuberculosis, M. bovis, M. africanum, M. microfti and M. canett. A cross sectional comparative study was conducted to assess the efficacy of the different diagnostic method of pulmonary tuberculosis of sputum samples from patients suspected of pulmonary tuberculosis. The study was conducted in the period from Feb 2015 to May 2016. Early morning sputum samples were collected in clean, sterile, leak proof, wide mouth containers and sent to the laboratory without delay. The processing of the sample carried out according to a bio-safety considerations. Each sample was subjected to Ziehl-Neelsen (ZN) staining and fluorescent Auramine-O (AO) staining, and then each sample was run on Gene Xpert, new clean, unscratched slide was labeled at one end with the laboratory number. Mucopurulent portion was used for smear preparation. An appropriate portion of the specimen transferred to the slide using a wooden-stick and smeared over an area of approximately 2 by 3 cm. A new slide for each specimen used. The smears allowed to air-dry for 15 minutes then fixed by passing over the flame 3 to 5 times for 3 to 4 seconds. Two hundred and forty one sputum specimens were examined by ZN staining technique , 52 (21.7%) of them were showed the characteristic of AFB appearance of serpentine cords under oil immersion field. Sixty two out of 241 (25.7%) sputum specimens using Fluorescent(AO) staining procedure showed the typical characteristic of AFB bacteria. Sixty seven out of 241(27.8%) were mycobacterium tuberculosis complex. In this study out of 241 specimens 67 (27.8%) were shown MTB detected on Gene Xpert while 52 (21.5%) were AFB positive by ZN staining and 62 (25.7%) were AFB positive when Fluorescent staining was used. The study concluded that as compared to AFB microscopy, Gene Xpert is more sensitive and specific not only for acid fast bacilli (AFB) detection but also for rifampacin (RIF) resistance. It also helps to avoid injudicious use of anti tuberculosis drugs.
Authors and Affiliations
Abdelbagi Eljayli Mohmmed Ahmed, Nuha Yousif, 2, Mohammed ELhassan, Ahmed Ibn Edriss, Ziyad Mudasir
Keywords
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- EP ID EP361541
- DOI 10.9790/0853-1702044955
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