Diagnostic Utility of Immunocytochemistry in Serous Effusions
Journal Title: International Journal of Medical Research Professionals - Year 2018, Vol 4, Issue 2
Abstract
Introduction: Cytodiagnosis of serous effusions relating to distinction between malignant and highly reactive mesothelial cells often possesses diagnostic challenge by routine diagnostic procedures. However, ancillary techniques like immunocytochemistry using panel of antibodies, help in increasing diagnostic accuracy. A combination antibody panel comprising of mesothelial and epithelial cell markers is suggested by various studies to provide distinction between malignant mesotheliomas and adenocarcinomas in serous effusions. Cell block in conjunction with immunohistochemistry can ease the process of more accurate diagnosis. Carletinin, a 29 kd calcium binding protein, normally expressed in neurons is a well recognised immunomarker of mesothelial cells. Materials and Methods: A total number of 504 cases of effusion cytology were studied during the period from August 2015 to August 2017. The fluids were first subjected to routine conventional staining procedures followed by staining with immunostains like carletinin and CEA in diagnostically difficult cases. Cell block preparations were done wherever possible. The results were calculated and tabled. Results: Out of the 504 cases studied, pleural effusion samples outnumbered peritoneal fluid samples. Tuberculosis was the most common cause of non - malignant effusions; adenocarcinoma of lungs and GIT were most commonly encountered entities in pleural and peritoneal fluid samples respectively. Conclusion: For more meaningful comparative studies, the combination of carletinin and CEA in differentiating reactive mesothelial cells and malignant cells were helpful. Utility of immunocytochemistry in cytodiagnosis of malignant effusion was highly significant as compared to the conventional smear methods.
Authors and Affiliations
Shilpa Dash, Gayatri Rath, Lity Mohanty
Keywords
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- EP ID EP537736
- DOI 10.21276/ijmrp.2018.4.2.008
- Views 78
- Downloads 0
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