Diagnostic value of methods for alder (Alnus incana) allergen sensitization determination in people with respiratory allergy
Journal Title: Туберкульоз, легеневі хвороби, ВІЛ-інфекція - Year 2019, Vol 0, Issue 2
Abstract
Objective — to study the parameters of diagnostic value (specificity and sensitivity) of skin prick testing with alder allergen and serologic determination of specific IgE to alder allergen among patients with respiratory allergy. Materials and methods. 88 patients with allergic rhinitis and/or atopic bronchial asthma were examined by three different methods of specific allergic diagnosis (in vivo and in vitro) in accordance with the guidelines of the ethics committee of the National Pirogov Memorial Medical University, all were beyond the acute period. The inclusion criteria were allergic rhinitis and/or atopic bronchial asthma diagnosis (both intermittent and persistent) with proven sensitivity to alder allergen. Skin prick test was carried out according to the classical testing procedure in accordance with regulatory documents with commercial extracts of allergens. Western blot testing for specific IgE levels was performed using RIDA qLine test systems (R-Biopharm AG, Darmstadt, Germany) and Euroline (Euroimmun). The sIgE concentration was converted to a nominal scale (grades) according to the following rules: < 0.35 IU/mL (level 0 — negative), 0.36—0.69 IU/mL (level 1 — boundary), 0.7—3.49 IU/mL (level 2 — slightly elevated), 3.50—17.4 IU/mL (level 3 — moderately elevated), 17.5—49, 9 IU/mL (level 4 — high levels), 50—100 IU/mL (level 5 — very high levels), and > 100 IU/mL (level 6 — extremely high levels). Results and discussion. In the examined patients, the sensitization to the alder allergen was 25.0 % (22 cases) by the presence of specific IgE by Rida Allergy Screen, 13.6 % (12 cases) by the presence of specific IgE by Euroline and 27.3 % (24 persons) according to the data of skin test by a blind test method with an appropriate allergen. The results of the analysis of the consistency of the results of two different methods of allergic diagnosis for the determination of sensitization to the alder through the construction of the confidence interval showed that the coefficient indicates a satisfactory agreement (r = 0.409) of the findings of the two different tests. Conclusions. The results of two serologic tests for determining the specific IgE to alder by Rida AllergyScreen and Euroline have a systematic difference in rates (1.4 kU/mL). Between the data of skin testing with alder allergen and detection of specific IgE by Rida AllergyScreen test, there is good agreement between the results, there is satisfactory agreement between the results of the research between the data of skin testing with alder allergen and the detection of specific IgE by the Euroline method.
Authors and Affiliations
A. Ye. Bogomolov
Keywords
Related Articles
- EP ID EP674597
- DOI 10.30978/TB2019-2-72
- Views 78
- Downloads 0
Complications of HIV infection
HIV-infected patients often develop various complications and comorbidities including opportunistic diseases that worsen patient’s general state and decrease antiretroviral treatment effectiveness. On the other hand, the...
Diagnostic value of methods for alder (Alnus incana) allergen sensitization determination in people with respiratory allergy
Objective — to study the parameters of diagnostic value (specificity and sensitivity) of skin prick testing with alder allergen and serologic determination of specific IgE to alder allergen among patients with respirator...
Tuberculosis and HIV co-infection in inpatients of phthisio-pulmonological profile: frequency, form of lesion, diagnostic features (according to data of highly specialized institution)
Objective — to clarify the prevalence of tuberculosis infection in HIV-positive inpatients of the TB hospital for 2014—2018 and features of its diagnosis in the affection of the chest cavity organs. Materials and method...
Condition of the proteolytic system in patients with tuberculous pleurisy
Recently, extrapulmonary forms of tuberculosis are increasingly common, which are not related to the pulmonary system. The largest proportion of extrapulmonary forms is pleural tuberculosis. This disease in case of delay...
Pathologic features of changes of digestive organs and nephros with isolated DRTB and co-infection DRTB/HIV/AIDS
Objective — based on а retrospective analysis to examine the features of pathological changes of the digestive system and nephros to improve clinical diagnosis of pathological conditions of these organs. Materials and...