Diagnostic value of methods for alder (Alnus incana) allergen sensitization determination in people with respiratory allergy
Journal Title: Туберкульоз, легеневі хвороби, ВІЛ-інфекція - Year 2019, Vol 0, Issue 2
Abstract
Objective — to study the parameters of diagnostic value (specificity and sensitivity) of skin prick testing with alder allergen and serologic determination of specific IgE to alder allergen among patients with respiratory allergy. Materials and methods. 88 patients with allergic rhinitis and/or atopic bronchial asthma were examined by three different methods of specific allergic diagnosis (in vivo and in vitro) in accordance with the guidelines of the ethics committee of the National Pirogov Memorial Medical University, all were beyond the acute period. The inclusion criteria were allergic rhinitis and/or atopic bronchial asthma diagnosis (both intermittent and persistent) with proven sensitivity to alder allergen. Skin prick test was carried out according to the classical testing procedure in accordance with regulatory documents with commercial extracts of allergens. Western blot testing for specific IgE levels was performed using RIDA qLine test systems (R-Biopharm AG, Darmstadt, Germany) and Euroline (Euroimmun). The sIgE concentration was converted to a nominal scale (grades) according to the following rules: < 0.35 IU/mL (level 0 — negative), 0.36—0.69 IU/mL (level 1 — boundary), 0.7—3.49 IU/mL (level 2 — slightly elevated), 3.50—17.4 IU/mL (level 3 — moderately elevated), 17.5—49, 9 IU/mL (level 4 — high levels), 50—100 IU/mL (level 5 — very high levels), and > 100 IU/mL (level 6 — extremely high levels). Results and discussion. In the examined patients, the sensitization to the alder allergen was 25.0 % (22 cases) by the presence of specific IgE by Rida Allergy Screen, 13.6 % (12 cases) by the presence of specific IgE by Euroline and 27.3 % (24 persons) according to the data of skin test by a blind test method with an appropriate allergen. The results of the analysis of the consistency of the results of two different methods of allergic diagnosis for the determination of sensitization to the alder through the construction of the confidence interval showed that the coefficient indicates a satisfactory agreement (r = 0.409) of the findings of the two different tests. Conclusions. The results of two serologic tests for determining the specific IgE to alder by Rida AllergyScreen and Euroline have a systematic difference in rates (1.4 kU/mL). Between the data of skin testing with alder allergen and detection of specific IgE by Rida AllergyScreen test, there is good agreement between the results, there is satisfactory agreement between the results of the research between the data of skin testing with alder allergen and the detection of specific IgE by the Euroline method.
Authors and Affiliations
A. Ye. Bogomolov
Keywords
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- EP ID EP674597
- DOI 10.30978/TB2019-2-72
- Views 80
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