Diagnostic value of vascular endothelial growth factor and interleukin-17 in association with molecular diagnosis of Wuchereria bancrofti infection
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2015, Vol 5, Issue 8
Abstract
Objective: To explore effective diagnosis of Wuchereria bancrofti through DNA-based techniques followed by assessment of vascular endothelial growth factor concentration (VEGF-C) and interleukin 17 (IL-17) as indicators for lymphatic endothelial cell activation, proliferation and massive tissue reaction that may be a good indicator for ongoing lymphatic filariasis. Methods: Blood samples were collected from 38 patients: 23 males (60.5%) and 15 females (39.5%) with filariasis and from controls (60 from a non-endemic and 22 from endemic areas). PCR was used to prove infection. A specific and sensitive ELISA was used to determine serum IL-17 and VEGF-C. Results: A total of 28 patients (46.7%) were positive by PCR, while 10 patients (16.7%) were negative by PCR. Serum level of vascular endothelial growth factor was significantly high in acute cases [(2 147.00 ± 556.00) pg/mL] and in cases of early elephantiasis [(1 950.00 ± 638.00) pg/mL] and lowest in cases of late elephantiasis, endemic and non endemic controls [(1 238.00 ± 443.00), (807.11 ± 6.20) and (857.00 ± 91.50) pg/mL respectively]. Serum IL-17 was found to be significantly high in acute cases, early elephantiasis and late elephantiasis cases [(8 601 ± 1131), (7 867 ± 473) and (6593 ± 378) pg/mL respectively] when compared to endemic controls [(3 194 ± 1 500 pg/mL)] and non endemic controls [(3 416 ± 1 101) pg/mL]. Conclusions: VEGF-C and its inducing factor IL-17 are expected to gain more importance in filariasis. Targeting such factors might ameliorate the pathology in chronic filariasis.
Pharmacognostic study and anti - inflammatory activity of Phyllanthus reticulatus Poir. fruit
Objective: To study pharmacognostic evaluation and anti - inflammatory activity of Phyllanthus reticulatus Poir. (P.reticulatus) fruit. Methods: The fruit sample was studied by macroscopical, microscopical and other WHO...
Molecular detection of hemolysin and aerolysin gene of Aeromonas spp. isolated from African tigrine frog (Hoplobatrachus occipitalis)
Objective: To determine the hemolysin (hlyA) and aerolysin (aerA) genes of Aeromonas sp. isolate collected from frog Hoplobatrachus occipitalis in Côte d’Ivoire. Methods: A total of 210 fresh healthy frogs and 384 smoked...
Therapeutic adherence: A prospective drug utilization study of oral hypoglycemic in patients with type 2 diabetes mellitus
Objective: To determine the drug utilization patterns and outcomes of treatment in terms of metabolic control in the type 2 diabetic patients on oral hypoglycemic agents in the outpatient department in the teaching hospi...
Predictors of scrub typhus: a study from a tertiary care center
Objective: To document the clinical and laboratory findings of scrub typhus infection and to determine the prognostic factors of the disease. Methods: Retrospective data were collected from 96 patients with scrub typhus...
Development of a multiplex polymerase chain reaction protocol for the simultaneous detection of Salmonella enterica serovar Typhi and Class 1 integron
Objective: To develop a multiplex polymerase chain reaction (PCR) protocol for the simultaneous detection of Salmonella enterica serovar Typhi (S. Typhi) and Class 1 integron, so as to aid rapid diagnosis of S. Typhi cas...