Diagnostic Yield of Cartridge-Based Nucleic Acid Amplification Test (CBNAAT) In Lymph Node Tuberculosis at Institute of Respiratory Disease, SMS Medical College, Jaipur
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2019, Vol 18, Issue 4
Abstract
Introduction: Lymph Node Tuberculosis (LNTB) is considered to be the local manifestation of a systemic disease. LNTB often affects children and young adults. Mycobacterial lymphadenitis most frequently affects patients in their second decade but may affect patients of any age. Some patients with LNTB may manifest systemic symptoms and these include fever, weight loss, fatigue, and night sweats. There are many methods for detecting LNTB in patients, but recently focus has been shifted to Molecular diagnosis or nucleic acid amplification (NAA). The purpose of the study was to evaluate the diagnostic yield of CBNAAT in LNTB. Materials And Methods: This study was conducted at Institute of Respiratory diseases, SMS Medical college, Jaipur, Rajasthan, India from June 2017, to June 2018. In total, 51 consecutive patients, with suspicion of LNTB were subjected fine needle aspiration (FNA) and the specimens were processed simultaneously for ZiehlNeelsen, Cytopathology, liquid culture via ‘NAP test’ or ‘TB Ag MPT4 Rapid’ immunochromatographic assay and CBNAAT. Results: Among 51 samples tested, CBNAAT detected DNA of MTBC in 37 samples (72.5%). Highest yield was found with cytopathology, with detecting caseous necrosis and AFB in 38 samples (86.4%). Standard biological assays, including AFB microscopy and culture, were positive, respectively, in 20 (39.2%) and 25 (49%). FNA CBNAAT sensitivity and specificity were assessed according to smear and culture results, clinical and cytopathological findings. The sensitivity and specificity of CBNAAT were 77.27% and 57.1% respectively. Conclusion: Combining FNAC and rapid genotypic diagnosis using automated systems (CBNAAT) should greatly improve access to appropriate diagnosis and treatment for patients with tuberculous lymphadenitis.
Authors and Affiliations
Vishnu Kumar Goyal, R. K. Jenaw
Keywords
Related Articles
- EP ID EP573262
- DOI 10.9790/0853-1804096367.
- Views 45
- Downloads 0
Traumatic Dislocation of the Hip Associated To Ipsilateral Femoral Shaft Fracture in a Racing Driver
The incidence of traumatic dislocation of the hip associated with femoral shaft fracture on the same side is rare. It meets with two main problems: diagnosis and treatment. We describe in this paper a special case which...
Role of Corticosteroids in Reducing Postoperative Morbidity Following Removal of Impacted Wisdom Teeth: A Comparative Study
Background: The surgical removal of impacted third molar teeth can result in considerable pain, swelling and trismus. Pain and swelling can be reduced via the glucocorticoids and with no nsteroidal anti inflammatory drug...
The Nutritional status of preschool children in urban and rural areas of Benin City, Edo State
The nutritional status of preschool children in urban and rural areas of Benin City, Edo State was investigated. Preschool children aged between 3 and 5 years were selected from nursery schools in urban (n= 150) and rura...
A Retrospective Study on Patients who Have Undergone Surgery For Goiter with Final Pathological Diagnosis of Hashimoto’s Thyroiditis
Hashimoto’s Thyroiditis is common cause of hypothyroidism with goiter in Iodine sufficient regions due to chronic auto immune disease. Patients with Hashimoto’s Thyroiditis present either a small diffuse goiter or marked...
Pure squamous cell carcinoma of the breast in a male
Primary squamous cell carcinoma of the breast is an extremely rare malignancy. It represents less than 0.1% of all primary invasive breast cancer. We present a case of pure squamous cell carcinoma of the breast in a male...