Differential Expression Pattern of Heat Shock Protein Genes in Toxigenic and Atoxigenic Isolate of Aspergillus flavus
Journal Title: Microbiology Research Journal International - Year 2016, Vol 14, Issue 2
Abstract
Aflatoxin biosynthesis in Aspergillus flavus requires coordinated expression of regulatory and structural genes. Aflatoxin production is optimum at 24-30°C and inhibition occurs at temperature higher than 35°C. Chaperones or heat-sock proteins are involved in processing of cellular protein and heat-stress induced protein, hence, we studied the genes encoding for heat-shock proteins under the influence of temperature (30°C vs. 37°C). A. flavus isolates, aflatoxigenic (MTCC9367) and atoxigenic (MTCC11580) were grown in glucose minimal salt broth for 24 hours for expression profile of selected genes using quantitative real-time PCR. We monitored the expression profile of genes encoding for heat-shock proteins (hsp98, hsp90, hsp70 and hsp60) and regulatory gene of aflatoxin biosynthesis pathway aflR. We found the similar trend for heat-shock proteins gene expression except hsp70 in aflatoxigenic and atoxigenic isolates of A. flavus. Expression for hsp70 was found to be upregulated at 30°C (vs 37°C)in atoxigenic isolate (P<0.001) of A. flavus in comparison of toxigenic (P<0.05) isolate. Since, heat-shock proteins are involved in protein folding and conformational stability of cellular proteins to maintain the biological activity, our data on transcripts encoding for heat-shock proteins suggested it may influence the aflatoxin biosynthesis process in A. flavus.
Authors and Affiliations
Raman Thakur, Shraddha Tiwari, Jata Shankar
Keywords
Related Articles
- EP ID EP350868
- DOI 10.9734/BMRJ/2016/25368
- Views 76
- Downloads 0
Molecular Detection of Antibiotic Resistance of Helicobacter pylori from Gastric Biopsies in Abidjan (Côte d'Ivoire)
Aims: To determine the genes of resistance to amoxicillin, clarithromycin and metronidazole of Helicobacter pylori in gastric biopsies in Côte d'Ivoire. Place and Duration: The study was performed at the department of g...
Molecular Characterization and Detection of Infection in Vector Snails of Urinary Schistosomiasis around Erinle and Eko Ende Dams in South West Nigeria
Aims: The prevalence of the schistosome cercariae in snail intermediate hosts has been known as one of the valuable predictors of the level of schistosomiasis transmission in different localities. This study was undertak...
Antimicrobial Resistance of Klebsiella pneumoniae -ESBL Producing Strains Isolated from Clinical Specimens in Abidjan (Cote de Ivoire)
Aims: The objective of this study is to detect the prevalence of qnr genes in broad-spectrum beta-lactamase producing Klebsiella pneumoniae, involved in hospital and community-acquired infections. Study Design: It is a...
Antimicrobial Properties of Ocimum sanctum and Calotropis gigantea Leaves
Objective: To evaluate antimicrobial and phytochemical activity of hexane and ethanolic extract of Ocimum sanctum and Calotropis gigantea leaves on clinical pathogens. Materials and Methods: The agar diffusion test was u...
Tolerance to Heavy Metals by Some Fungal Isolates from Petroleum Refinery Effluent in Kaduna, Nigeria
Investigation was carried out to assess the potential of some fungal species to tolerate and grow in biomass in vitro in the presence of some heavy metals (HMs) (Pb, Zn, Cr and Cd) from Kaduna Refinery and Petrochemical...