Direct detection of rpoB and katG gene mutations of Mycobacterium tuberculosis in clinical samples
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2017, Vol 7, Issue 8
Abstract
Objectives: To study the rpoB and katG gene mutation rate and its markers. Methods: Cross-sectional study methods were used to study Tuberculosis. A total of 45 sputum samples were collected from Annapurna Neurological Institute and Allied sciences. Then, acid fast bacilli staining were performed. Positive and negative samples were carried for conventional polymerase chain reaction identification and electrophoresis. Results: Out of 45 samples, 3 were acid fast bacilli positive and the rest were negative. Male participants were more as compare to female participants and the mutation in rpoB and katG gene was found similar i.e. 6.66% among the total samples. Conclusions: We can conclude that genetic mutation in Mycobacterium tuberculosis can be identified directly from the clinical samples. However, we have carried this study in less sample size and to validate research on large number of sample is recommended.
Authors and Affiliations
Sunil Pandey, Ashima Lamichhane, Anu Byanjankar, Ansuma Kharel, Chandrakala Rai, Sunil Prasad Lekhak, Menuka Karki
Keywords
Related Articles
- EP ID EP269695
- DOI 10.1016/j.apjtb.2017.07.018
- Views 60
- Downloads 0
Toxicological evaluation and protective effect of ethanolic leaf extract of Launaea taraxacifolia on gentamicin induced rat kidney injury
Objective: To evaluate the toxic potential of Launaea taraxacifolia leaf extract (LTE) in rats within 14 d of oral administration and also assess the potential of LTE in protecting against kidney injury induced by gentam...
Effect of crocin carotenoid on BDNF and CREB gene expression in brain ventral tegmental area of morphine treated rats
Objective: To investigate the effect of crocin carotenoid on BNDF and CREB gene expression in the brain ventral tegmental area (VTA) and the serum level of BDNF in morphine-treated rats compared to control. Methods: In t...
Assessment of co-segregated TLR4 genotypes among Nigerian children with asymptomatic and clinical malaria
Objective: To assess the occurrence and pattern of Toll-like receptor 4 (TLR4) cosegregated genotypes among children with Plasmodium falciparum malaria in Nigeria. Methods: In this case-control study, a total of 79 Plasm...
Anti-cancer and anti-inflammatory activities of aronia (Aronia melanocarpa) leaves
Objective:To determine the anti-cancer effect of aronia leaf extract on SK-Hep1 cells using migration, metallo metrix proteinase-2/-9 (MMP-2/-9) and MT-1 MMP expression and to evaluate the anti-inflammatory activities...
Effect of morin, a flavonoid against DOCA-salt hypertensive rats: a dose dependent study
Objective To determine the protective effect of morin, a flavonoid against deoxycorticosterone acetate (DOCA)-salt induced hypertension in male Wistar rats. Methods Hypertension was induced in uninephrectomized rats b...