Direct somatic embryogenesis in Swietenia macrophylla King
Journal Title: Biotecnología Vegetal - Year 2006, Vol 6, Issue 2
Abstract
Swietenia macrophylla King is difficult to be propagated by tissue culture and there is not an efficient system via organogenesis, due to problems of microbial contamination, phenolic oxidation and death of tissue in the phase of in vitro establishment of explants. In order to establish a protocol for obtaining somatic embryos, zygotic embryos were used as initial plant material. Three combinations of 2,4-D with kinetin were studied, to obtain the formation of somatic embryos. After six weeks of culture, the number of explants with high and low somatic embryogenesis frequency were determined. So that the somatic embryos in globular stage reach the final stages of torpedo and cotyledonal, these were placed in three treatments with 6-BAP (0.2, 0.4 y 0.6 mg.l -1 ). The number of somatic embryos that reached the torpedo and cotyledonal stages were evaluated after 30 days of culture. Results demonstrated that direct somatic embryogenesis from immature zygotic embryos is obtained in the culture medium composed by MS salts with 4.0 mg.l -1 of 2,4-D and 1.0 mg.l -1 of kinetin. Higher percentage of somatic embryos in cotiledonal stage (91.7 %), was obtained with 0.4 mg.l -1 of 6-BAP.
Authors and Affiliations
Raúl Collado, Raúl Barbón, Daniel Agramonte, Felipe Jiménez-Terry, Martha Pérez, Odalys Gutiérrez
Keywords
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