Direct somatic embryogenesis in Swietenia macrophylla King
Journal Title: Biotecnología Vegetal - Year 2006, Vol 6, Issue 2
Abstract
Swietenia macrophylla King is difficult to be propagated by tissue culture and there is not an efficient system via organogenesis, due to problems of microbial contamination, phenolic oxidation and death of tissue in the phase of in vitro establishment of explants. In order to establish a protocol for obtaining somatic embryos, zygotic embryos were used as initial plant material. Three combinations of 2,4-D with kinetin were studied, to obtain the formation of somatic embryos. After six weeks of culture, the number of explants with high and low somatic embryogenesis frequency were determined. So that the somatic embryos in globular stage reach the final stages of torpedo and cotyledonal, these were placed in three treatments with 6-BAP (0.2, 0.4 y 0.6 mg.l -1 ). The number of somatic embryos that reached the torpedo and cotyledonal stages were evaluated after 30 days of culture. Results demonstrated that direct somatic embryogenesis from immature zygotic embryos is obtained in the culture medium composed by MS salts with 4.0 mg.l -1 of 2,4-D and 1.0 mg.l -1 of kinetin. Higher percentage of somatic embryos in cotiledonal stage (91.7 %), was obtained with 0.4 mg.l -1 of 6-BAP.
Authors and Affiliations
Raúl Collado, Raúl Barbón, Daniel Agramonte, Felipe Jiménez-Terry, Martha Pérez, Odalys Gutiérrez
Keywords
Related Articles
- EP ID EP684124
- DOI -
- Views 123
- Downloads 0
Bacterial canker caused by Clavibacter michiganensis subsp. michiganensis in naranjilla in Ecuador
Bacterial canker of naranjilla or lulo (Solanum quitoense and Solanum pectinatum) is a new disease that could completely destroy naranjilla plants. The objectives of this study were to identify and characterize bacteri...
Biotechnological tools against Fusarium oxysporum f. sp. cubense Tropical race 4 in Musa spp.
Panama disease caused by Fusarium oxysporum f. sp. cubense (Foc) is one of the most damaging diseases attacking Musa worldwide. Race 1 caused an epidemic that destroyed the banana export industry based cultivar ‘Gros M...
Selection of guava clonal lines of the cultivar Enana roja (EEA 18-40) for use in genetic improvement and propagation
The present work was carried out with the objective of evaluating and selecting in field conditions clonal lines of guava (variety Enana Roja, EEE 18-40) according to the fruits characters. The plants used were obtained...
Optimal concentration of Ethyl Methane Sulfonate for phenotypic variation induction in Phaseolus vulgaris L. cv. ‘DOR 364’
Common bean (Phaseolus vulgaris L.) is the main legume used for human consumption worldwide and is an important objective in breeding programs to favor certain characteristics of agronomic interest. In order to determi...
Donor plant mycobiota and filamentous fungi contaminants from the in vitro establishment of five species forest
Although protocols to propagate large numbers of woody species by in vitro culture have been developed, microbial contamination of explants is a fundamental problem which limits its application. The objectives of this st...