Disease diagnosis with electrophoretic analysis of urinary protein and comprehensive urinary proteome research
Journal Title: Journal of Analytical Bio-Science - Year 2011, Vol 34, Issue 2
Abstract
The importance of urine has tended to be underestimated given its image of an unnecessary waste of biological fluid. Recently, large-scale projects have been established to analyze urinary proteins, more than 1500 of which have been identified as candidate biomarkers. Urinary proteins are routinely analyzed using electrophoresis. Even one-dimensional electrophoresis is considered a useful tool for the diagnosis of diseased conditions. This section describes a successful diagnostic application using urinary protein profiles analyzed by cellulose acetate membrane electrophoresis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In the contextof the standardization for a urinary proteome, the sampling and storage of urine are also shown. Finally, we discuss current examples of urinary biomarker research in renal diseases.
Authors and Affiliations
Kiyoko Shiba
Keywords
Related Articles
- EP ID EP150218
- DOI -
- Views 104
- Downloads 0
Examination of the properties of a monoclonal anti-fibronectin antibody MO
The properties of an anti-fibronectin monoclonal antibody MO which recognizes an epitope on a subpopulation of human neutrophils were examined. The result of an ELISA examination showed that MO specifically bound to immo...
Prevention of lifestyle-related diseases in school children
Lifestyle-related diseases, including dyslipidaemia, hypertension, hyperinsulinaemia, and obesity, are recognized as major risk factors for cardiovascular disease in adults. There is convincing evidence that lifestyle-re...
Effect on Quality of Life of a Lentinus edodes mycelia-enriched diet (L･E･M®) in patients with terminal cancer
Purpose: It is important to offer the highest possible quality of life (QOL) for terminal cancer patients in palliative medicine. It was examined whether Lentinus edodes mycelia (L･E･M®) -containing diet (CORELEM®) was e...
The new technology to improve specificity of immunochromatography
Immunochromatography is one of the assays which are well known and used in clinical setting. To compare with the other assay, its rapidness, easy handling and inexpensive cost are being mentioned for its advantages. Howe...
New creatine kinase assay kit for clinical analyzers- Know-How and techniques for reagent stabilization -
Creatine kinase (CK) is an essential enzyme that is present in various tissues like skeletal muscle, myocardium or brain, and it plays a crucial role in energy metabolism. During muscle or myocardial injury, CK is releas...