Disease diagnosis with electrophoretic analysis of urinary protein and comprehensive urinary proteome research
Journal Title: Journal of Analytical Bio-Science - Year 2011, Vol 34, Issue 2
Abstract
The importance of urine has tended to be underestimated given its image of an unnecessary waste of biological fluid. Recently, large-scale projects have been established to analyze urinary proteins, more than 1500 of which have been identified as candidate biomarkers. Urinary proteins are routinely analyzed using electrophoresis. Even one-dimensional electrophoresis is considered a useful tool for the diagnosis of diseased conditions. This section describes a successful diagnostic application using urinary protein profiles analyzed by cellulose acetate membrane electrophoresis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In the contextof the standardization for a urinary proteome, the sampling and storage of urine are also shown. Finally, we discuss current examples of urinary biomarker research in renal diseases.
Authors and Affiliations
Kiyoko Shiba
Keywords
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