DISINFECTION OF EXTRACTED HUMAN TEETH: A COMPARATIVE STUDY
Journal Title: Journal of Advanced Medical and Dental Sciences Research - Year 2017, Vol 5, Issue 1
Abstract
Background: Extracted teeth are used commonly by dental students as well as researchers to improve their technical skills for future dental practice as well as to conduct various studies. Extracted teeth are a potential source of infection and should be disinfected/ sterilized before use. Aim: To assess the efficacy of different materials for disinfection/sterilization of extracted human teeth. Settings and Design: The study was conducted in A.J Institute of medical/dental sciences, ethical clearance was obtained from the institutional ethical committee. Material and Methods: A total of 90 extracted non carious human teeth were kept in nine disinfectant media-10 % formalin, 99% acetic acid, vinegar (commercially available), 6% hydrogen peroxide, neem extract, curcumin extract, aloe vera extract, lime extract and normal saline. Teeth were kept in different groups for one week, samples were collected and streaked over the surfaces of Mc-Conkeys and Blood agar medium. The media were later incubated at 37 ͦ C for 48 hours. No growth in the culture media was considered an effective method of sterilization/disinfection. Statistical analysis: Chi Square Analysis. Results: 10% formalin, 6% hydrogen peroxide and 99% acetic acid were totally effective. The result was statistically significant with a Chi square value of 80.957 and P < 0.001 for 7 days and X2 value of 180.00 and P < 0.001 for 3 days. Conclusions: 10 % formalin, 6% hydrogen peroxide and 99% acetic acid can be used as an disinfectant medium for extracted human teeth
Authors and Affiliations
Tony Paul, Dinkar Desai, Mohammed Shahid, Niloufa Z Aziz, Gomes Leslie, Bhakthi Sadhu
Keywords
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