Distribution and multi-drug resistance pattern of escherichia coli isolated from patients in federal medical center, Owerri
Journal Title: Scientific Journal of Microbiology - Year 2013, Vol 2, Issue 9
Abstract
This study was carried out to investigate the distribution and multi-drug resistance (MDR) pattern of Escherichia coli isolated from patients in Federal Medical Centre (FMC), Owerri, Imo State. 255 clinical specimens were collected, including urine, stool, wound swabs, high vaginal swabs, urethral swabs, endocervical swabs, sputum and aspirates. Specimens were cultured on MacConkey agar (MA, Oxoid) and Eosin Methylene Blue Agar (EMB, Oxoid). 100 E. coli isolates were obtained. A significantly high rate of infection was found in females (p<0.05). Susceptibility testing was done on 50 selected isolates using Disc diffusion method with Mueller-Hinton agar. Antibiotics used were Amoxicillin/Clavulanic acid- AMC (30µg), Cefpirome- CPO (30µg), Cefpodoxime- CPD (10µg), Cefotaxime- CTX (30µg), Cefoxitin- FOX (30µg), Ciprofloxacin- CIP (5µg), Tetracyclin- TE (30µg), Nalidixic acid- NA (30µg), Chloramphenicol- C (30µg), Ceftriaxone- CRO (30µg) and Sulphanethoxazole/Trimethoprim- SXT (25µg). Ceftriaxone and Ciprofloxacin had the highest percentage sensitivity (92.0%), while Nalidixic acid and Tetracyclin were the least sensitive (8.0%). Four isolates (8.0%) were resistant to all antibiotics used. This study established that Ceftriaxone and Ciprofloxacin are the most effective drugs for the treatment of E. coli infections in Owerri. However, it is advised that these drugs be taken only on prescription to prevent development of drug resistance.
Authors and Affiliations
N. C. J. Anyanwu*| Department of Microbiology, Faculty of Sciences, Ahmadu Bello University, Zaria, Nigeria., W. C. John| Federal College of Forestry, Jos, Plateau State, Nigeria., C. A. Nsofor| Madonna University, Okija, Elele Campus, Rivers State, Nigeria.
Keywords
Related Articles
- EP ID EP1173
- DOI 10.14196/sjm.v2i9.798
- Views 484
- Downloads 24
Nutritional potentials of sugarcane peels meal on nutrients intake and digestibility of Yankasa sheep
This study was conducted to evaluate the nutritional potentials of sugarcane peels on nutrients intake and digestibility of Yankasa Sheep. Twenty (20) male growing Yankasa lambs aged 12 – 18 months, with an average initi...
Sweat Based Blood Glucose Analysis
Painless and reliable glucose monitoring for diabetes is the battle of the next generation. As technology advances, both diagnostic techniques and equipment are designed to work non-invasively. Glucose monitoring become...
Human bocavirus frequency in pre-school and school-aged hospitalized wheezing children and association to epidemiological risk factors of wheezing
Recurrent wheezing in infancy is associated with acute respiratory infection (ARI) and atopy. The objectives of this work were to determine the frequency of respiratory human bocavirus (HBoV) in children hospitalized wit...
Microbial quality of selected sandwiches sold at retail outlets of fast food shops in Dhaka city and Mymensingh town of Bangladesh
the experiment was designed to undertake a study on the sanitary quality of commercial fast foods sold at retail outlets of fast food shops in Dhaka city and Mymensingh town of Bangladesh. One hundred sandwiches were sub...
Serum lipid profile and hepatic dysfunction in moderate plasmodium falciparum infection
Plasmodiumfalciparum is one of four distinct species of the malaria parasite that afflicthumans and pose a threat to public health. The present study seeks to ascertainthe serum lipid profile and associated hepatic dysfu...