Distribution and multi-drug resistance pattern of escherichia coli isolated from patients in federal medical center, Owerri
Journal Title: Scientific Journal of Microbiology - Year 2013, Vol 2, Issue 9
Abstract
This study was carried out to investigate the distribution and multi-drug resistance (MDR) pattern of Escherichia coli isolated from patients in Federal Medical Centre (FMC), Owerri, Imo State. 255 clinical specimens were collected, including urine, stool, wound swabs, high vaginal swabs, urethral swabs, endocervical swabs, sputum and aspirates. Specimens were cultured on MacConkey agar (MA, Oxoid) and Eosin Methylene Blue Agar (EMB, Oxoid). 100 E. coli isolates were obtained. A significantly high rate of infection was found in females (p<0.05). Susceptibility testing was done on 50 selected isolates using Disc diffusion method with Mueller-Hinton agar. Antibiotics used were Amoxicillin/Clavulanic acid- AMC (30µg), Cefpirome- CPO (30µg), Cefpodoxime- CPD (10µg), Cefotaxime- CTX (30µg), Cefoxitin- FOX (30µg), Ciprofloxacin- CIP (5µg), Tetracyclin- TE (30µg), Nalidixic acid- NA (30µg), Chloramphenicol- C (30µg), Ceftriaxone- CRO (30µg) and Sulphanethoxazole/Trimethoprim- SXT (25µg). Ceftriaxone and Ciprofloxacin had the highest percentage sensitivity (92.0%), while Nalidixic acid and Tetracyclin were the least sensitive (8.0%). Four isolates (8.0%) were resistant to all antibiotics used. This study established that Ceftriaxone and Ciprofloxacin are the most effective drugs for the treatment of E. coli infections in Owerri. However, it is advised that these drugs be taken only on prescription to prevent development of drug resistance.
Authors and Affiliations
N. C. J. Anyanwu*| Department of Microbiology, Faculty of Sciences, Ahmadu Bello University, Zaria, Nigeria., W. C. John| Federal College of Forestry, Jos, Plateau State, Nigeria., C. A. Nsofor| Madonna University, Okija, Elele Campus, Rivers State, Nigeria.
Keywords
Related Articles
- EP ID EP1173
- DOI 10.14196/sjm.v2i9.798
- Views 493
- Downloads 24
Prevalence of haemoparasites in red sokoto goats slaughtered at Ahiaeke Market, Umuahia, Abia State, Nigeria
The prevalence of haemoparasites in Red Sokoto goats slaughtered at Ahiaeke market, Umuahia, Abia State Nigeria were determined between the months of September and November 2014. Blood samples were collected from 125 app...
Prevalence of brucella abortus antibodies in bovine serum from gusau modern abattoir, Zamfara state, Nigeria
A study for bovine brucellosis was conducted using serology to determine the status of the disease in slaughtered cattle. Three hundred and twenty (320) sera were collected from Gusau Modern Abattoir, Zamfara State. The...
Mycological investigation in patients with acute otitis media
Middle ear swabbed samples from 272 patients with acute otitis media (AOM) attending Ear, Nose and Throat clinics were collected and cultured using standard mycological technique. Deoxyribonuclease and In-vitro susce...
Antimicrobial effect of Satureja bachtiarica extracts aqueous, ethanol, methanol and glycerin on streptococcus pyogenes, pseudomonas aeruginosa and staphylococcus epidermidis
The Iranian medicinal plants, such as Satureja bachtiarica have been utilized as traditional medicines by the indigenous people of Chaharmahal va Bakhtiari in Iran. In this study, Satureja bachtiarica were dried in suita...
Effect of agrochemicals on microflora in soybean rhizospheric soil
After application of agrochemicals at 1, 30 DAS and at harvest the total number of fungi, bacteria, actynomycetes, pseudomonas, azatobactor, rhizobium were counted.The result with regard to bacterial population in soybea...