Variation in light intensity air temperature and relative air humidity leads to diurnal variations of photosynthetic rate and leaf relative water content. In order to determine the diurnal changes in net photosynthetic rate of vine plants and influence of the main environmental factors gas exchange in the vine leaves were measure using a portable plant CO2 analysis package. The results show that diurnal changes in photosynthetic rate could be interpreted as single-peak curve with a maximum at noon (10.794 µmol CO2 m-2 s-1). Leaf relative water content has maximum value in the morning; the values may slightly decrease during the day (day of June with normal temperature no rain no water restriction in soil).