DNA fingerprinting and molecular diversity analysis for the improvement of brinjal (Solanum melongena L.) cultivars
Journal Title: Journal of Advanced Biotechnology and Experimental Therapeutics - Year 2018, Vol 1, Issue 1
Abstract
An investigation was conducted to assess genetic diversity of Bangladesh Agricultural Research Institute (BARI) released 11 varieties of brinjal (BARI begun 1, BARI begun 4-10, BARI hybrid begun 1, 3 and 4) using PCR-based randomly amplified polymorphic DNA (RAPD) markers. A total of 44 distinct DNA amplified bands were observed for five primers (OPB-04, OPB-08, OPD-02, OPP-13 and OPW-08) with an average of 5.6 polymorphic bands per primer. The overall gene diversity was detected 0.216 and level of polymorphism was found 63.64%. The pair wise inter-variety similarity indices ranged from 67.03% to 97.61%. The UPGMA dendrogram segregated 11 genotypes of brinjal into two main clusters. The first major cluster had only one genotype (BARI begun 6) and the second major cluster had rest of ten genotypes. BARI begun 6 vs BARI begun 1 showed the highest Nei’s genetic distance (0.5261) as they are released from different parental origin. On the other hand, BARI begun 9 vs BARI begun 7 varietal pair showed lowest genetic distance (0.0465) as they are released from same parental origin. The experiment reveals genetic diversity and relatedness among 11 brinjal genotypes which may be informative for the future varietal identification and genetic improvement of this vegetable crop.
Authors and Affiliations
Sharmin Sultana, Mohammad Nazrul Islam, Md Ekramul Hoque
Keywords
Related Articles
- EP ID EP36332
- DOI -
- Views 448
- Downloads 0
A reliable and easy method to isolate a pure population of bovine granulosa cells from slaughterhouse ovaries for in vitro studies
In a growing follicle, granulosa cells (GCs) are the major cellular component and perform several important functions. GCs are an excellent choice for in vitro experimentation to understand their functions in vivo. How...
Herbal contraceptive effect of Abrus precatorius, Ricinus communis, and Syzygium aromaticum on anatomy of the testis of male Swiss albino mice
The present research was designed to investigate in vivo herbal contraceptive effects on the anatomy of the testis in male Swiss albino mice (Mus musculus) and to observe that whether the herbal extracts depart any bal...
Analysis of telomere length in the coronary artery to determine the pathogenesis of coronary artery disease
Endothelial dysfunction is one of the earliest pathological features in atherosclerosis. The current study designed to analyse the telomere length of human coronary artery derived endothelial cells to identify the rela...
Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testicular damage in Swiss albino mice
Lead toxicity is the vital issue in the developing countries that causes serious health hazards in animals and humans. Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testic...
Prophylactic effects of vitamin E and selenium on di (n-butyl) phthalate-induced testicular damage in prepubertal rats
Di (n-butyl) phthalate (DBP), a potential endocrine disruptor, adversely affects male reproductive function via activation of oxidative stress. This study was conducted to investigate the protective effects of vitamin...