DOSE AND STAGE SPECIFIC EFFECTS OF THYROXINE ON THE TADPOLES OF DUTTAPHRYNUS MELANOSTICTUS (ANURA: BUFONIDAE)
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 8
Abstract
Objective: Aim of the study was to investigate the effects of thyroxine on the pre metamorphic tadpoles of the common Asian toad, Duttaphrynus melanostictus (Anura: Bufonidae), an ideal model for understanding the role of thyroxine during vertebrate development.Methods: Tadpoles of two developmental stages, i.e., Gosner stege 30 and 34 was exposed to 50, 100 and 200 ng/ml of thyroxine by adding the hormone to the tadpole rearing water. Toxic and teratogenic effects of thyroxine on the tadpoles were recorded. Besides, effects of thyroxine on specific activity of acid phosphatase, a lysosomal marker enzyme in the tails of the tadpoles were investigated.Results: A Dose and stage specific toxic effect of the hormone was observed in the treated tadpoles. There was precocious metamorphosis with incomplete differentiation of limbs, deformities of body and tail in the treated tadpoles. Biochemical investigation of tails showed a dose specific elevation in the specific activity of acid phosphatase up to 2.5 fold in 50 ng/ml and 3.15 fold in 100 ng/ml treated tadpoles as compared to corresponding control tadpoles.Conclusion: Thus, the hormone thyroxine exhibited toxic as well as teratogenic effects on the developing tadpoles and also led to dose dependent elevation in specific activity of acid phosphatase in vivo in their tails. Keywords: Acid phosphatase, Duttaphrynus melanostictus, Tail regression, Thyroxine
Authors and Affiliations
Cuckoo Mahapatra, Anamika Patro, Pravati Kumari Mahapatra
Keywords
Related Articles
- EP ID EP578287
- DOI -
- Views 66
- Downloads 0
EFFECTS OF A. MELLIFICA EXTRACT ON THE HEMATOLOGICAL, BIOCHEMICAL, URINE AND HISTO-PATHOLOGICAL PARAMETERS OF WHITE ALBINO RABBITS
Objective: To observe the effects of Apis mellifica extract on different hematology, biochemistry and histopathology parameters in rabbits for determining its safety and efficacy.Method: Hematological, biochemical invest...
METHOD DEVELOPMENT AND VALIDATION OF NORETHINDRONE ACE-TATE ASSAY AND ITS RELATED IMPURITIES IN API AND PHARMACEUTICAL FORMULATION WITH ORTOGONAL DETECTOR TECHNQUES
Objective: To develop and validate a sensitive and stability indicating gradient reverse phase ultra-high performance(UHPLC-PDA) liquid chromatography with photodiode array(PDA) and mass spectroscopy (MS) of Norethindron...
PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT POTENTIAL OF TRIBULUS TERRESTRIS FRUIT AND MESUA FERREA FLOWER EXTRACTS: A COMPARATIVE STUDY
Objective: This study aimed to investigate the antioxidants and free radicals scavenging properties of ethanolic extracts from Tribulus terrestris fruit (TTF) and Mesua ferrea flower (MFF).Methods: The antioxidant and fr...
ANTIOXIDANT, ANTIHYPERGLYCEMIC AND ANTIGLYCATION PROPERTIES OF SOME SWERTIA SPECIES FROM WESTERN GHATS
Objective: Oxidative stress and Advanced Glycation End-products have been associated with diabetic complications. Therefore, natural compounds or extracts that possess both antioxidant and anti-glycation activities might...
SOLUBILITY ENHANCEMENT OF KETOCONAZOLE VIA SALT AND COCRYSTAL FORMATION
Objective: Pharmaceutical salt and cocrystal is a promising alternative method for improving the solubility and dissolution rate of active pharmaceutical ingredients. In this work, an attempt was made to improve solubili...