DOSE DEPENDENT CARDIAC EFFECTS OF DOXORUBICIN IN WISTAR RATS: A BIOCHEMICAL AND HISTOPATHOLOGICAL ANALYSIS
Journal Title: International Journal of Pharmaceutical Sciences and Research (IJPSR) - Year 2019, Vol 10, Issue 6
Abstract
Although doxorubicin is used widely in animal models to induce cardiotoxicity, serial dose-dependent cardiac effects have not been investigated in experimental animal models to date. Therefore, the objective of this study was to find out the dose-dependent changes of doxorubicin-induced acute cardiotoxicity, both biochemically and histopathologically in Wistar rats. Wistar rats were divided into nine groups. Group 1: normal control; Groups 2-9: eight doses of doxorubicin (13, 14, 15, 16, 17, 18, 19 & 20 mg/kg, intraperitoneal injection, after 16 h fast). Animals were sacrificed on the 4th day, and blood was collected for the estimation of cardiac troponin I (cTnI), aspartate aminotransferase (AST) and superoxide dismutase (SOD) activities and heart tissues were collected for histopathological assessment. Mean cTnI concentrations of groups 1-9 were 0, 39.46 ± 1.8, 60.92 ± 2.7, 87.79 ± 1.8, 116.96 ± 2.7, 147.79 ± 2.3, 163.96 ± 1.5, 197.38 ± 2.3 and 221.54 ± 1.8 pg/mL respectively. In groups 1-9, mean AST activities were 38.90 ± 0.98, 42.23 ± 0.99, 44.07 ± 1.20, 50.77 ± 1.70, 57.93 ± 3.40, 58.54 ± 0.65, 63.57 ± 0.69, 68.94 ± 2.20 and 79.83 ± 1.20 U/L and SOD activities were 77.73 ± 0.86, 72.92 ± 1.3, 66.49 ± 0.91, 54.06 ± 0.92, 28.01 ± 0.45, 21.12 ± 0.56, 16.12 ± 0.46, 14.34 ± 0.70 and 8.75 ± 0.94% respectively. Significant differences (P<0.05) between group 1 and groups 2-9 were evident in all three diagnostic parameters. Degree of histopathological damage due to doxorubicin increased with increasing dosage of doxorubicin.
Authors and Affiliations
J. A. N. Sandamali et al.
Keywords
Related Articles
- EP ID EP580639
- DOI 10.13040/IJPSR.0975-8232.10(6).2700-10
- Views 106
- Downloads 0
A COMPREHENSIVE REVIEW ON COSTUS PICTUS D. DON
Costus pictus is rhizomatous medicinal herb, commonly known as “Insulin plant,” well known for its therapeutic properties. Many important and beneficial aspects of C. pictus are reported, which includes anti-diabetic, an...
UNCOMMON SIDE EFFECTS OF COMMONLY USED ANTI-DIABETICS: TIME TO MONITOR THEM
Diabetes, a chronic endocrine metabolic disorder, is managed using several anti-diabetic medications. Being a chronic disease requiring lifelong medications, the long-term safety profile and patient acceptance are crucia...
DRUG UTILIZATION PATTERN IN GERIATRIC PATIENTS OF GENERAL MEDICINE IN A SECONDARY CARE HOSPITAL
The purpose of the research was to assess the prescribing pattern in geriatrics and to analyze the prescription using World Health Organization (WHO) core prescribing indicators and American Geriatric Society (AGS) Beers...
SYNTHESIS OF NEFOPAM ANALOGUES AND CHARACTERISATION
Modified Benzoxazocine moiety of Nefopam with N-Protected-ethanolamine, fluorobenzene benzylbromide, 2,3-dimethyl-benzene, naphthalene, fluro-naphthalene. In these modification Friedel Craft alkylation plays vital role w...
ANTIBACTERIAL, ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF BACTERIAL CAROTENOIDS ISOLATED FROM RHODOPSEUDOMONAS PALUSTRIS KRPR01 AND KRPR02
The microbial pigments have more applications than synthetic pigments and are easily biodegradable and safe to use. Among all bacteria, the anoxygenic phototrophic purple non-sulfur bacteria have more applications and ca...