DOSE DEPENDENT CARDIAC EFFECTS OF DOXORUBICIN IN WISTAR RATS: A BIOCHEMICAL AND HISTOPATHOLOGICAL ANALYSIS
Journal Title: International Journal of Pharmaceutical Sciences and Research (IJPSR) - Year 2019, Vol 10, Issue 6
Abstract
Although doxorubicin is used widely in animal models to induce cardiotoxicity, serial dose-dependent cardiac effects have not been investigated in experimental animal models to date. Therefore, the objective of this study was to find out the dose-dependent changes of doxorubicin-induced acute cardiotoxicity, both biochemically and histopathologically in Wistar rats. Wistar rats were divided into nine groups. Group 1: normal control; Groups 2-9: eight doses of doxorubicin (13, 14, 15, 16, 17, 18, 19 & 20 mg/kg, intraperitoneal injection, after 16 h fast). Animals were sacrificed on the 4th day, and blood was collected for the estimation of cardiac troponin I (cTnI), aspartate aminotransferase (AST) and superoxide dismutase (SOD) activities and heart tissues were collected for histopathological assessment. Mean cTnI concentrations of groups 1-9 were 0, 39.46 ± 1.8, 60.92 ± 2.7, 87.79 ± 1.8, 116.96 ± 2.7, 147.79 ± 2.3, 163.96 ± 1.5, 197.38 ± 2.3 and 221.54 ± 1.8 pg/mL respectively. In groups 1-9, mean AST activities were 38.90 ± 0.98, 42.23 ± 0.99, 44.07 ± 1.20, 50.77 ± 1.70, 57.93 ± 3.40, 58.54 ± 0.65, 63.57 ± 0.69, 68.94 ± 2.20 and 79.83 ± 1.20 U/L and SOD activities were 77.73 ± 0.86, 72.92 ± 1.3, 66.49 ± 0.91, 54.06 ± 0.92, 28.01 ± 0.45, 21.12 ± 0.56, 16.12 ± 0.46, 14.34 ± 0.70 and 8.75 ± 0.94% respectively. Significant differences (P<0.05) between group 1 and groups 2-9 were evident in all three diagnostic parameters. Degree of histopathological damage due to doxorubicin increased with increasing dosage of doxorubicin.
Authors and Affiliations
J. A. N. Sandamali et al.
Keywords
Related Articles
- EP ID EP580639
- DOI 10.13040/IJPSR.0975-8232.10(6).2700-10
- Views 133
- Downloads 0
PHYTOCHEMICAL ANALYSIS AND IN-VITRO ANTICANCER ACTIVITY OF DURANTA ERECTA L. (VERBENACEAE)
The aim of the present work is the evaluation of anticancer activity of Duranta erecta L. stem extracts and also an investigation of the phytoconstituents from the different extracts of the plant. Chloroform, ethyl aceta...
EVALUATION OF IN-VITRO ANTIOXIDANT AND ANTICANCER ACTIVITY OF MONOCHORIA VAGINALIS LEAVES ON Hep2 AND HeLa CELL LINES
The entire plant (Monochoria vaginalis (Burm. f.) C. Presl.) except root is being used as a vegetable and has been used in traditional Indian system of medication for treating various diseases. But there are not many sci...
PREVALENCE OF COGNITIVE IMPAIRMENT IN METABOLIC SYNDROME PATIENTS IN URBAN INDIA
The association between metabolic syndrome and cognitive impairment is not well established. The aim of the present study was to estimate the prevalence of cognitive impairment in adults with metabolic syndrome (MetS). M...
PHYTOCHEMICAL STUDY OF INDONESIAN CURCUMA ROTUNDA RHIZOME AND ITS ANTIOXIDANT ACTIVITY TOWARDS 1,1-DIPHENYL-2-PICRYLHYDRAZYL (DPPH)
Curcuma rotunda, locally known as temu kunci, has been reported containing flavonoids in its rhizome that exhibit antioxidant activity. This study aimed to isolate secondary metabolites from the polar fraction of acetone...
METHOD DEVELOPMENT, VALIDATION AND STABILITY INDICATING ASSAY ON GLIMEPIRIDE IN TABLET DOSAGE FORM BY RP-UFLC
The study aimed to develop a specific, exact, selective, precise and accurate Reversed-Phase Stability Indicating Ultra-fast Liquid Chromatography (RP-UFLC) strategy is created and validated for the determination of glim...