METHOD DEVELOPMENT, VALIDATION AND STABILITY INDICATING ASSAY ON GLIMEPIRIDE IN TABLET DOSAGE FORM BY RP-UFLC
Journal Title: International Journal of Pharmaceutical Sciences and Research (IJPSR) - Year 2019, Vol 10, Issue 9
Abstract
The study aimed to develop a specific, exact, selective, precise and accurate Reversed-Phase Stability Indicating Ultra-fast Liquid Chromatography (RP-UFLC) strategy is created and validated for the determination of glimepiride in the tablet dosage form. The method showed an adequate separation for glimepiride from their degradation products. The optimum separation was achieved by using 250 mm × 4.6 mm C18 column (5 μm) with a mixture of potassium dihydrogen orthophosphate adjusted to pH 4.6 using (orthophosphoric acid) and acetonitrile in the ratio of 75:25 at a flow rate of 1 ml/min. The detection was carried out at 236 nm, and the retention time was found to be 5.9 min. Linearity was observed (correlation coefficient r2 0.9989). % R.S.D was found to be less than 2%. Accuracy of the method found to be in the range of 98.0 to 102.00 (% w/w). The above method was validated concerning system suitability, linearity, precision, the limit of detection (LOD) and limit of quantification (LOQ), accuracy (recovery) and robustness according to ICH guidelines. The linearity of the above methods was found to be 2-10 µg/ml for glimepiride and force degradation were carried out. Hence these methods can be used for routine analysis in quality control laboratories.
Authors and Affiliations
R. Maruthi et al.
Keywords
Related Articles
- EP ID EP627599
- DOI 10.13040/IJPSR.0975-8232.10(9).4345-53
- Views 96
- Downloads 0
IMPACT OF PETROCHEMICAL EFFLUENT ON HEMATOLOGICAL PARAMETERS IN THE FRESHWATER FISH, LABEO ROHITA
The present investigation was carried out the effect of petrochemical effluent on hematological parameters in freshwater fish, Labeo rohita using the standard method. The petrochemical effluent has the potential to cause...
PHYTOCHEMICAL STUDY OF INDONESIAN CURCUMA ROTUNDA RHIZOME AND ITS ANTIOXIDANT ACTIVITY TOWARDS 1,1-DIPHENYL-2-PICRYLHYDRAZYL (DPPH)
Curcuma rotunda, locally known as temu kunci, has been reported containing flavonoids in its rhizome that exhibit antioxidant activity. This study aimed to isolate secondary metabolites from the polar fraction of acetone...
EFFECT OF PHARMACIST DIRECTED COUNSELLING SERVICES ON KNOWLEDGE, ATTITUDE, AND PRACTICE (KAP) AND BLOOD PRESSURE CONTROL IN HYPERTENSIVE PATIENTS: A RANDOMIZED CONTROL TRIAL
Pharmacists play a vital role in providing counseling to the hypertensive patients to understand disease, risk factors, medications, and non-pharmacological measures required in controlling Blood Pressure (BP). This stud...
EFFECT OF PIPER NIGRUM (LINN.) ON INFERTILITY INDUCED BY ETHIONAMIDE AND PARA AMINO SALICYLIC ACID IN MALE SPRAGUE-DAWLEY RATS
Tuberculosis is a global problem. The tuberculosis is the common cause of male infertility in developing countries due to the use of second-line anti-tuberculosis drugs (ATB). The experiments were conducted at Unique Bio...
A QUEST FOR BETTER PROBIOTIC DELIVERY AND FRONTIERS OF PROBIOTIC APPLICATION IN MEDICAL SCIENCE: A CRITICAL REVIEW
Probiotics are live microorganisms that when introduced orally positively contribute to the activity of the intestinal microflora and the health of its host. Various studies showed that probiotics are potentially able to...