DRUG EFFECT ON LEUKEMIA CELLS THROUGH SINGLE CELL ASSAY METHOD
Journal Title: IJAR-Indian Journal of Applied Research - Year 2019, Vol 9, Issue 1
Abstract
Acute myeloid leukemia (AML) is a cancer of the myeloid line of blood cells that is characterized by accumulation of immature malignant cells in the bone marrow. Leukemia cells can hide in bone marrow niche or vascular niche. So,many of the chemical drugs cannot kill these cells. One of the greatest challenges in Acute Myeloid Leukemia (AML) treatment is to prevent relapsing. AMD3100 is used as one of the most common chemical drugs that can inhibit this signal. In the current study, the effect of AMD3100 on the adhesion property of leukemia cells on stromal cells can be investigated by using engineering tools, namely, Optical tweezers (OT) and Dielectrophoresis (DEP), to probe single cell property. AMD3100 not only inhibits the CXCR4/SDF-1α signal pathway but also reduces gene expression of CXCR4 and VLA4 on leukemia cells. This drug also softens leukemia cells. This work provides a new way to investigate cell behavior under drug treatment. The use of combined engineering tools will benet drug discovery and assessment for leukemia treatment.
Authors and Affiliations
Naufa N N
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