Drug Susceptibility Testing of M. tuberculosis to Isoniazid and Rifampicin- A Comparison between MTT Tube Method and LJ Proportion Method
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2015, Vol 9, Issue 2
Abstract
Aim: To evaluate the methodology of MTT tube assay and compare it with standard proportion method for detection of drug susceptibility of M. tuberculosis to rifampicin (RIF) and isoniazid (INH). Study Design: Prospective. Place and Duration of Study: Sher-i-Kashmir Institute of Medical Sciences, Kashmir, India. One year study. Methodology: MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] assay was performed on 60 clinical isolates of M. tuberculosis. An inoculum of 107CFU/ml prepared in Middlebrook 7H9 with OADC (Oleic acid, albumin, dextrose and catalase) was chosen as standard. For each drug three tubes were used, one drug containing (INH 0.2 µg/ml or RIF 1 µg/ml), second inoculum control and third blank control. The method was performed after incubating the tubes at 37°C for 4 days for RIF and 7 days for INH. Results were read visually and by spectrophotometer at 570 nm. Relative optical density units of 0.2, was taken as cutoff. Results of drug susceptibility were compared with those obtained by Lowenstein Jensen proportion method. Results: For RIF, sensitivity was 88.9% and 94.4%; specificity was 100% and 97.6% for visual MTT and MTT by RODU respectively. For INH similar sensitivity of 95.1% was seen while specificity was 97.0% and 95.0% by visual MTT and MTT by RODU respectively. There was almost perfect agreement between proportion and MTT method for both drugs. Turn-around time for MTT assay was 7 days. Conclusion: The MTT tube assay can be used for rapid drug susceptibility testing of M. tuberculosis to RIF and INH.
Authors and Affiliations
Roomi Yusuf, Tehmeena Wani, Gulnaz Bashir, Nargis Bali
Keywords
Related Articles
- EP ID EP347374
- DOI 10.9734/BJMMR/2015/18688
- Views 50
- Downloads 0
Moringa oleifera Enriched Diet Ammeliorates the Toxic Effects of Palm Wine and Local Gin (Ogogoro) on Some Biochemical Parameters in Albino Rats
The effect of Moringa oleifera enriched diet on the toxic effects of palm wine and local gin on liver enzymes, lipid profile and electrolytes in albino rats was determined in this research. Methodology: Thirty female al...
Honey: Nature’s Wonder of Healing
Aim: Aim of this present study is to quantify, evaluate and assess the therapeutic benefits of honey on the treatment of recurrent apthous ulcer. Materials and Methods: Sixty patients with minor oral ulcers were attended...
Management of Sodium Hypochlorite Accident: A Case Report
Among root canal disinfection liquids, sodium hypochlorite stands out due to its antimicrobial and tissue dissolving properties. During use, diligent care should prevent extrusion into periapical areas. In spite of preca...
Association of Blood Group O with Gastrointestinal Bleeding in Continuous-Flow LVAD Patients
Background: Increasing use of mechanical circulatory devices for advanced heart failure therapy has led to challenges in managing complications that arise from device implantation for long term support either as a bridge...
Measurments of Bolton’s Tooth Width Ratios in Karnataka Population: A Preliminary Study
Aim: This study was conducted to establish the Bolton’s ratios’ norms ((The mean Overall Ratio (OR) and Anterior Ratio (AR) norms)) in 100 Karnataka subjects and to compare it with the overall ratio and anterior ratio of...