Dynamics of biomass and carotinogenesis activity Rhodotorula glutinis (fresenius) F. C. Harrison (1982) after the effect of ultraviolet
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2017, Vol 9, Issue 2
Abstract
Yeast Rhodotorulaglutinis is characterized by a high ability to form various valuable biologically active substances, including carotenoids. This large group of pigments is widely used in various industries, including aquaculture to stimulate the development of caviar and larvae, normalizing the life of adult individualshydrobionts. Among other microorganisms, yeasts Rhodotorula are characterized by growth rate, the ability to digest easily and hardly accessible carbon substrates, the unpretentiousness to the mineral composition of the nutrient medium, and reproduction in low pH values. At present, an active search for methods for intensifying the synthesis of pigment compounds by microorganisms and cheapening their production is underway. In this paper, the method of physical mutagenesis is tested by ultraviolet radiation of type C with a length of 254 nm. Exposure of microorganisms by ultraviolet radiation was carried out after 48-hour cultivation on solid medium by bactericidal lamps in sterile cells for 120 minutes at a distance of 40 cm from the surface of the medium. Under the action of UV-C, the culture of Rhodotorulaglutinis, which has a high level of carotenoid synthesis activity. It was shown that after irradiation the intensity of accumulation of biomass of yeast decreased by 1.5 times. The content of β-carotene in modified strain cells increased by 25%, the concentration of tolulodone was 69% higher than that of native culture, and torulin was found in non-intestinal quantities. At the same time, changes in the individual characteristics of the colonies were noted compared to the native strain. Among them: the appearance of bright orange or bright pink color (as opposed to pale orange in the native strain), the matte nature of the surface of the colonies (glossy for native) and accelerated appearance of pigmentation.
Authors and Affiliations
Іванна Краєвська, Лілія ВАСІНА
Keywords
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- EP ID EP303883
- DOI 10.31861/biosystems2017.02.183
- Views 64
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