ECO-FRIENDLY APPROACH FOR LUMINESCENCE DETECTION OF BENDAMUSTINE HYDROCHLORIDE USING GOLD NANOPARTICLES IN THE PRESENCE OF ORGANIZING SOLUTION
Journal Title: World Journal of Pharmaceutical Research - Year 2017, Vol 6, Issue 2
Abstract
The present approach, introduced a simple, sensitive and reliable ecofriendly spectrofluorimetric method for the determination of bendamustine hydrochloride. The described method is mainly based on the enhancement effect of green synthesized gold nanoparticles (AuNPs) on the fluorescence intensity of bendamustine hydrochloride in the presence of (1.0% w/v) anionic micellar solution, sodium dodecyl sulfate. The fluorescence intensity was detected at λem 368 nm after λex at 310 nm. The pH was adjusted using phosphate buffer of pH = 6. The proposed method displayed a good linear relationship over a concentration range of 0.1-100 μg mL-1 with regression coefficient (r = 0.999) and lower limits of detection and quantitation of 0.023 μg mL-1 and 0.074 μg mL-1, respectively. The current method is validated according to ICH guidelines and it is successfully applied to the analysis of bendamustine hydrochloride in intact powder and its pharmaceutical preparations with high accuracy. The percentage recoveries were found to be (99.7±0.4, % RSD = 0.4 % and n = 6) and (99.4±0.5, % RSD = 0.5 and % n= 6) for intact drug and parenteral injection, respectively. No significant difference between the obtained results with those obtained from other published method.
Authors and Affiliations
Dr. Maha F. El-Tohamy
Keywords
Related Articles
- EP ID EP617233
- DOI -
- Views 195
- Downloads 0
SAFETY AND IMMUNOGENICITY OF SEASONAL TRIVALENT INACTIVATED INFLUENZA VACCINES IN PREGNANT WOMEN
Pregnant women are at high risk for influenza virus infection and serious influenza-related complications and are recommended to receive inactivated influenza vaccine during the influenza season. Before 2009, although ma...
PHARMACOLOGICAL ACTIVITY OF Psidium guajava LEAVES - A REVIEW
Plants for thousands of years have been used to enhance health and for medicinal purposes. Psidium guajava is one which has an enormous wealth of medicinal value. One of such nutritionally fit and commonly available plan...
EFFECTS OF PROCESSED CAJANUS CAJAN (PIGEON PEA) SEED ON LIPID PROFILE IN ALBINO WISTAR RATS
This study was designed to evaluate the nutritional effect of processed Cajanus cajan seeds on lipid profile of albino wistar rats. Sixty-four (64) (disease free stock) of albino rats were used. The rats were randomly as...
THE MEDICAL MYCOLOGY AND THE LEGACY OF ANTONIO GONZALEZ OCHOA
This work presents an approach of the scientific work of Dr. Antonio González-Ochoa, prominent Mexican mycologist and dermatologist. His research had a deep transcendence in the national and international medical mycolog...
A REVIEW ON: A PHARMACOLOGICAL PROPERTIES OF ABELMOSCHUS ESCULENTUS
Abelmoschus esculentus L. is commonly known as lady's fingers, bhindi, okra or gumbo, is a flowering plant belongs to family Malvaceae. Okra is a multipurpose crop due to its various uses of the fresh leaves, buds, flowe...