Ecofriendly, Economic Surrogative for Xylene
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 3
Abstract
Background: Xylene is an aromatic hydrocarbon, mostly used as a clearing agent during tissue processing and as a de-waxing agent during histopathological staining. The biohazardous nature ofxylene, makes it a potential occupational hazard for histopathological technicians. Exposure to xylene can occur via inhalation, ingestion or direct contact with skin, eyes etc. Exposure and handling sections of xylene is maximum during dewaxing of sections. These have led to the question of a substitute agent which is less toxic and safer. Aim: To assess the efficacy of 1.5% of dishwashing liquid and 1.5% of detergent liquid as a deparaffinizing agent for H&E staining technique. Objective:To utilize eco-friendly, economic substitute for xylene. Materials and Methods: Using 20 paraffin embedded tissue blocks, three sections were prepared and considered into 3 groups as Group A, Group B, Group C. Group A was stained with conventional H&E method, Group B was stained using 1.5% of dishwashing liquid as a deparaffinizing agent and Group C was stained using 1.5% of detergent liquid as a deparaffinizing agent. Statistical Analysis: ANOVA test was used to calculate the test of significance. Result and Conclusion:1.5% of dishwashing liquid and 1.5% of detergent liquid are comparatively an effective alternative for xylene thus acting as anecofriendly, economic surrogative for xylene.
Authors and Affiliations
Janani Ilango, M. Devi, D. Vijayalakshmi .
Keywords
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