Effect of Additive Tamoxifen Citrate to SMART Medium on Human Sperm Parameters during In vitro Sperm Activation
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 4
Abstract
This study was aimed to investigate the effect of addition of tamoxifen citrate on the outcome of in vitro human sperm activation. One hundred semen samples for fertile and infertile men were randomly collected from the subjects at the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies / AlNahrain University. The mean age was (32.21± 0.83) years and mean duration of infertility was (5.26 ± 0.41) years. Before using centrifugation swim-up technique, the washed samples were divided into three groups: control group (G1) without tamoxifen citrate, while in the second(G2) and third(G3) group, two concentrations oftamoxifen citrate (20µg/mL and 40µg/mL) respectively were added post-activation enriched with SMART medium. Macroscopic examination of semen included: Semen liquefaction time, semen pH, semen viscosity and semen volume, while microscopic examination of semen included: sperm concentration, motility, morphology, agglutination and cells other than spermatozoa. The results showed significant (P<0.05) increased in sperm motility, progressive sperm motility and normal sperm morphology of post-activation as compared with preactivation. Among treated groups, a significant (P<0.05) decreased was found in sperm concentration, immotile sperm and total progressive sperm, while a significant (P<0.05) increased was observed in progressive sperm motility and normal sperm morphology in (G3) as compared with others groups. Based on the results of this study, it can be concluded that the addition of 40µg/mL of tamoxifen citrate to washed sperms can improve sperm motility.
Authors and Affiliations
Noor Kareem Kadhim, Jabaar H. Yenzeel1*, Muhammed-Baqir M-R Fakhrildin**
Keywords
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- EP ID EP390039
- DOI 10.9790/3008-1204038791
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