DAN damage analysis in liver of male albino rats treated with different doses of cyclophosphamide
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 2
Abstract
Cyclophosphamide is a synthetic alkylating agent chemically related to the nitrogen mustards widely used as an anticancer and immunosuppressive drug.This study was to investigate the damage in the DNA liver of male albino rats treated with low and high dose of cyclophosphamide.Use for this purpose 30 of the strain Sprague Dawley albino rats and divided into three groups, each group consisted of 10 rats; Group 1 (control) was injected with physiological solution (normal saline 0.9%), Group 2 (low dose) received 50mg/kg/b.w/weekly of drug and Group 2 (high dose) received 80mg/kg/b.w/weekly of the drug for 10 weeks.At the end of dosage, duration, the rats were dissected and the liver was excised and washed with normal saline.DNA damage analysis by using comet assay according comet assay kit. This result of comet assay showed that damage in DNA of liver cells at 10 weeks of treatment with cyclophosphamide high and low dose in four parameters comet high, comet medium, comet low and no damage. With low and high dose groups increase in comet high, comet medium percentage, decrease in comet low, and no damage percentage. The results of the present study indicated that high and low dose of Cyclophosphamide able to induce DNA damage in the liver of male albino rats
Authors and Affiliations
Saif. S. Abd alhassan, Dr. Abd H. Baraj
Keywords
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- EP ID EP395020
- DOI 10.9790/3008-1302061417
- Views 72
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