EFFECT OF ANTIBIOTICOTHERAPY ON THE TOTAL AMOUNT OF OXALATE-DEGREADING BACTERIA IN THE RATS INTESTINAL TRACT
Journal Title: Мікробіологія і біотехнологія - Year 2019, Vol 0, Issue 2
Abstract
Aim. To determine the quantity of oxalate-degrading bacteria (ODB) in fecal biopsy and mucosa-associated microbiota of the colon and small intestine of rats. Methods. Study was conducted on male Wistar rats (weight 170–200 g, n=7). The object of the study was the change in the total amount of oxalate-degrading microbiota of the fecal and mucosa-associated biotope of the colon and small intestine. The mix of ampicillin (75 mg/kg) and metronidazole (50 mg/kg) was injected once a day, for 3 days per os. The dynamics of microbiota changes were evaluated immediately after the antibiotics withdrawals, on the 18th, 29th and 59th days of the experiment. The microbiological analysis of fecal (CFU/g) and mucosa-associated (CFU/cm2 ) biotope of rats was carried out bacteriologically by sowing the according dilutions on elective media. The results are presented in the form of M±m lg CFU/g (cm2 ). Results. The number of bacteria from the fecal biopsy sowed in a highly selective Oxalate Medium (5 g/l Na2 C2 O4 ) decreased from 6,22±0,20 to 4,37±0,80 lg CFU/g (Р<0,05) at 29th day of experiment and remained below the values in the corresponding control group on the 59th day of the experiment. A significant decline in the amount of oxalate-degrading microbiota (from 7.05±0.35 to 5.13±0.05 lg CFU/cm2, P<0.05) was recorded in the mucosa-associated microbiota of the colon on the 59th day of the experiment. However, in the mucosa-associated microbiota of the small intestine, their number was within the control values. The number of anaerobic saccharolytic bacteria of the genera Lactobacillus and Bifidobacterium remained within the control values in the fecal and mucosa-associated biotopes of the colon and small intestine after antibiotics treatment. Conclusion. Progressive decrease in the total number of oxalate-degrading bacteria during antibiotic-associated dysbiosis can become the cause of oxalate concretions formation.
Authors and Affiliations
I. V. Akulenkо, М. Y. Korbush, V. O. Stetskа, T. M. Serhiychuk, G. M. Tolstanova, N. M. Stepanovа
Keywords
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- EP ID EP670072
- DOI 10.18524/2307-4663.2019.2(46).159163
- Views 80
- Downloads 0
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