Effect of antibiotics on Agave fourcroydes Lem in vitro propagation
Journal Title: Biotecnología Vegetal - Year 2016, Vol 16, Issue 1
Abstract
High microbial contamination on henequen (Agave fourcroydes Lem) in vitro propagation reduces its efficiency. This work aimed to determine the effect of the use of antibiotics in the control of bacterial contaminants of this culture. Bacterial contaminants were identified and their susceptibility to different antibiotics it were determined. The two best-acting antibiotics were added to the propagation medium and the number of explants contaminated with bacteria and necrotics was quantified. The antibiotic and concentration that did not cause phytotoxicity to the explants and where the lowest percentage of contamination was obtained it was used to continue the propagation of the plants. These were transferred to the acclimatization stage and at 30 days of culture the number of live plants and the number of roots per plant were quantified. In addition, the length of the roots (cm) was measured and the leaf area was calculated. Micrococcus spp., Pseudomonas spp., Agrobacterium spp., Bacillus subtilis and Bacillus licheniformis were found. The antibiotics tested inhibited the in vitro growth of the isolated contaminants and the best results were obtained with Ticar and Cefotaxime. Added to the plant propagation medium, Ticar was phytotoxic over 50 mg l -1 and cefotaxime could be used at 100 mg l -1 without causing damage to the explants. The results showed that the plants that came from the culture medium with cefotaxime 100 mg l -1 showed a significant increase of the variables evaluated in the acclimatization stage.
Authors and Affiliations
Enildo Abreu, Maryla Sosa del Castillo, Gerardo Ascunce del Sol, Gerardo González
Keywords
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