EFFECT OF APIUM GRAVEOLENS EXTRACTS ON CASPASE-3, -8, -9 AND APAF-1 IN LNCaP CELLS
Journal Title: Kocatepe Medical Journal - Year 2024, Vol 25, Issue 3
Abstract
OBJECTIVE: In this study it was aimed to investigate the anti-cancer effect of ethanolic extracts of apium graveolens (celery) plant on cell viability, caspases involved in apoptosis and apoptosis protease activation factor-1 (Apaf-1) as inducer/suppressor in prostate cancer cell line LNCaP cells. MATERIAL AND METHODS: LNCaP cell line was used in our study. LNCaP cells were divided into 4 groups treated with ethanolic extracts of apium graveolens at increasing concentrations ranging from 0, 1500, 2000, 2500 μg/mL. The indicated extract concentrations were applied to LNCaP cells separately for 24 and 48 hours. Caspase-3, -8, -9 and Apaf-1 were measured by ELISA method from the cell lysate prepared at the end of the treatment. The results were expressed as ng/ml in proportion to the number of viable cells. RESULTS: Treatment of LNCaP prostate cancer cells with apium graveolens extracts for 24 and 48 hours did not cause a significant difference in caspase-3 and caspase-9 levels. However, caspase-8 levels were significantly increased at 2000 and 2500 μg/ml extract concentrations and Apaf-1 levels were significantly increased at 2500 μg/ml extract concentration in a dose- and time-dependent manner compared to the control groups at 24 and 48 hours. CONCLUSIONS: The results of this study show that ethanolic extracts of apium graveolens plant have anticancer and apoptotic properties by increasing caspase-8 and Apaf-1 levels in LNCaP prostate cancer cell line. Further in-vitro and in-vivo studies are needed to support our findings.
Authors and Affiliations
Halit Buğra Koca Tülay Köken Tülay Akan
Keywords
Related Articles
- EP ID EP740658
- DOI https://doi.org/10.18229/kocatepetip.1340255
- Views 46
- Downloads 0
THE MEDIATOR ROLE OF PERSONALITY TRAITS IN THE RELATIONSHIP BETWEEN MEDICAL AND DENTISTRY STUDENTS' ANXIETY AND ANATOMY ACHIEVEMENT - GENITAL MODULE EXAMPLE
OBJECTIVE: While studying the anatomy of the genital system in medical education, students may feel embarrassed and anxious due to the anatomical features of this system. This feeling of anxiety, combined with students'...
INVESTIGATION OF THE RELATIONSHIP OF DL-PHENYLALANINE AND DL-ALANINE WİTH THE HEDGEHOG PATHWAY IN THE PROSTATE CANCER CELL LINE (PC-3)
OBJECTIVE: In this study, it was aimed to investigate the effect of DL-Phenylalanine and DL-Alanine on the Hedgehog signaling pathway on the PC-3 prostate cancer cell line. MATERIAL AND METHODS: The effects of DL-Phenyl...
CLINICAL AND DEMOGRAPHIC CHARACTERISTICS OF FAMILIAL MULTIPLE SCLEROSIS IN THE INNER AEGEAN REGION
OBJECTIVE: Multiple sclerosis (MS) is a common autoimmune demyelinating disease of the central nervous system (CNS). In this study, it was aimed to collect patients with MS retrospectively, to compare the characteristics...
CLINICAL SIGNIFICANCE OF HEMATOLOGICAL PARAMETERS IN CHILDREN WITH PANCREATITIS
OBJECTIVE: In this study, we aimed to retrospectively evaluate the significance of new hematological parameters in the diagnosis of the inflammatory process of acute pancreatitis in children diagnosed with acute pancreat...
DETERMINATION OF NURSING STUDENTS' ATTITUDES TOWARDS STIGMATIZATION BY OTHERS FOR SEEKING PSYCHOLOGICAL HELP FOR THEIR MENTAL SYMPTOMS
OBJECTIVE: To determine the mental symptoms of nursing students and their attitudes towards stigmatization by their close circle when seeking psychological help. MATERIAL AND METHODS: The population of the research, whi...