Effect of BAP and ANA on the in vitro multiplication of Bambusa vulgaris var. Schrad vulgaris. ex Wendl.
Journal Title: Biotecnología Vegetal - Year 2009, Vol 9, Issue 3
Abstract
Bambusa vulgaris var. vulgaris Schrad. ex Wendl. is a bamboo species of great economic importance. Developing protocols for propagation via organogenesis and somatic embryogenesis would be an efficient alternative to propagate this species since it adapts well to the soil and climatic conditions of Cuba. The aim of this work was to determine the effect of different concentrations of 6-BAP (1.0, 3.0 and 6.0 mg.l -1 ) and NAA (0, 0.5, 1.0 mg.l -1 ) on B. vulgaris in vitro multiplication. Number of shoots and leaves per explant were quantified. Also, height of plants was measured on the twentieth day of culture. Multiplication of Bambusa vulgaris var. vulgaris in liquid culture medium with BAP was achieved. Combination of BAP and NAA in the culture medium was not effective for this purpose. The result solves one of the main limitations to use biotechnology techniques in this crop.
Authors and Affiliations
Yudith García-Ramírez , Marisol Freire-Seijo, Blanca Pérez, Ortelio Hurtado
Keywords
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