Influence of growth regulators and physical state of the culture medium on the in vitro multiplication of Colocasia esculenta clone 'INIVIT MC2001'
Journal Title: Biotecnología Vegetal - Year 2013, Vol 13, Issue 4
Abstract
To achieve adequate results in the in vitro multiplication of taro cultivars is required to standardize the culture conditions. The aim of this study was to determine the influence of growth regulators and physical state of the culture medium on in vitro multiplication of Colocasia esculenta clone ‘INIVITC2001’. Five treatments involving indole acetic acid (IAA) and 6-benzylaminopurine (6-BAP) were established: 1.0 mg l -1 IAA + 3.0 mg l -1 6-BAP, 3.0 mg l -1 6-BAP, 4.0 mg l -1 6-BAP, 5.0 mg l -1 6-BAP, 1.0 mg l -1 IAA + 6.0 mg l -1 6-BAP. After 30 days of culture the multiplication coefficient was calculated. Furthermore, the effect on the physical state of the culture medium on this variable during three subcultures was determined. When the culture medium consisting of MS salts and vitamins with 4.0 mg l -1 of 6-BAP was used the greater multiplication coefficient was obtained. Handling the physical state of the culture medium for three subcultures influenced the multiplication coefficient in each subculture, in average coefficient multiplication of three subcultures and the total number of explants obtained after three subcultures. With the culture medium in semisolid state better growth and higher explants multiplication coefficient was achieved.
Authors and Affiliations
Diosdada Galvez Guerra, Manuel Cabrera Jova, Yoel Beovides, Ania Robaina Jiménez, Sergio Rodríguez, Daniel Rodríguez
Keywords
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