Mycobiota of donor plants and fungal contaminants from the in vitro establishment of bamboo species
Journal Title: Biotecnología Vegetal - Year 2008, Vol 8, Issue 1
Abstract
The presence of fungal contaminants may limit the establishment of bamboos explants. Knowing the contaminant microorganisms present in donor plants allow to trace more appropriate control strategies. This work was aimed to identify the mycobiota of donor plants of bamboo species and to identify the filamentous fungi in the establishment stage to control them. The method of the humid camera was used to determine and to identify the filamentous fungus. Then, direct preparations to the optic microscope of growth fungus were carried out. It was observed that the donor plants of bamboos had a mycobiota composed of diverse generous of filamentous fungi: Alternaria, Botryotrichum, Cladosporium, Curvularia, Fusarium y Nigrospora. The genera Asteromella, Bipolaris and Humicola were identified in the establishment stage, while Alternaria, Botryotrichum, Cladosporium, Curvularia, Fusarium and Nigrospora appeared in donor plants and in the establishment. In species Dendrocalamus asper, Dendrocalamus strictus, Guadua angustifolia and Gigantochloa atter genera Nigrospora showed the highest frequency of appearance, while in Bambusa vulgaris the filamentous fungus with highest frequency was Fusarium. A phytosanitary defense plan for donor plants was developed. It included systemic and contact fungicides which spectrum of activity covered the identified fungi genera.
Authors and Affiliations
Mayra Acosta-Suárez, Yelenys Alvarado-Capó, Mileidy Cruz-Martín, Berkis Roque, Cynthia Sánchez-García, Michel Leiva-Mora, Marisol Freire-Seijo, Yudith García-Ramírez, Zaida Pérez, Teresa Salabarría, Marisol Tejeda, Milagros González, Ortelio Hurtado
Keywords
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