Influence of the in vitro environment on the germination of somatic embryos of Coffea arabica L. cv. ‘Caturra rojo’ and Clematis tangutica K.
Journal Title: Biotecnología Vegetal - Year 2017, Vol 17, Issue 2
Abstract
The in vitro environment is a factor that in recent years has begun to investigate, because gases such as oxygen, carbon dioxide and ethylene play an important role in the morphogenesis of somatic embryos and their development in plants. The objective of this work was to determine the effect of the CO 2 on the germination of coffee somatic embryos (Coffea arabica L. cv. ‘Caturra rojo’) and clematis (Clematis tangutica K.). Three gas mixtures composed of CO 2 concentrations (2.5, 5.0 and 10.0%) combined with 21% O 2 and two controls (passive exchange and forced ventilation) were used. A positive effect of CO 2 on the germination of somatic embryos in the torpedo stage in coffee and clematis was obtained, because in the treatments with passive exchange, where there was CO 2 accumulation, germination of the somatic embryos was superior to the treatments with Forced ventilation. With 2.5% and 5.0% CO 2 , the germination process is stimulated while with 10.0% CO 2 there is an inhibition of germination with the appearance of malformations and hyperhydricity.
Authors and Affiliations
Raúl Barbon , Walter Preil , Alina Capote , Elio Jiménez
Keywords
Related Articles
- EP ID EP683032
- DOI -
- Views 170
- Downloads 1
Lavandula angustifolia L. plants regeneration from in vitro leaf explants-derived callus as conservation strategy
Lavandula angustifolia L. is an aromatic and medicinal herb with multiple industrial applications. Nevertheless, the over exploitation of wild plantation attempts against to its conservation as natural resource. The aim...
Establishment of sugarcane mother plant bank in semicontroled conditions for in vitro propagation
The propagation of sugarcane (Saccharum spp.) Requires seed with physiological and phytosanitary quality. The work was carried out with the objective of establishing a bank of sugarcane mother plants under semi-controlle...
Rhizosphere of three cultivars of Phaseolus vulgaris L. source of endospore-forming bacteria with biotechnological potential
Plant Growth Promoting Rhizobacteria constitute a promising source of microorganisms, with the capacity to develop new biopesticides that contribute to pest control, with an agroecological and sustainable basis. The obje...
Callus formation and establishment of cell suspensions sugarcane embryogenics from leaf segments in vitro plants
The somatic embryogenesis, propagation technique and tool for the genetic improvement in sugar cane (Saccharum spp. hybrid), has presented multiple inconveniences that the regeneration protocols described in the litera...
Effect of selenite and inulin on the Capsicum annuum L. - Phytophthora capsici interaction in greenhouse
Capsicum annuum L. is a crop of great economic importance in Mexico and is severely affected by the wilt caused by Phytophthora capsici. The objective of this work was to determine the effect of selenite and inulin on th...