Establishment of sugarcane mother plant bank in semicontroled conditions for in vitro propagation
Journal Title: Biotecnología Vegetal - Year 2021, Vol 21, Issue 1
Abstract
The propagation of sugarcane (Saccharum spp.) Requires seed with physiological and phytosanitary quality. The work was carried out with the objective of establishing a bank of sugarcane mother plants under semi-controlled conditions to guarantee the efficiency of in vitro propagation with plants free of Xanthomonas albilineans. A greenhouse was modified and the structure was remodeled, a Sarán shade mesh (50%) and a sprinkler irrigation system were placed. The plants were grown in plastic containers (20 m3 volume) with a mixture of cachaça compost and zeolite 3:1. Stems of cultivars C98-357, C97-445 and C86-156, without symptoms of leaf scald and with six months of culture, were cut into segments with a bud. Hydrothermal treatment was applied and subsequently the biostimulant VIUSID Agro® (0.8 ml l-1) before the buds planting. At three months of culture, a serological diagnosis was made to detect the presence of X. albilineans. The negative plants were treated with biostimulants and the response of the in vitro establishment was evaluated. Adequate plant growth was achieved under the conditions described. The establishment of a bank of sugarcane mother plants under semi-controlled conditions and its management guaranteed that microbial contamination and phenolic oxidation decreased in the in vitro establishment phase. The effectiveness of the hydrothermal treatment combined with the semi-controlled conditions and the management of the plants make it possible to reduce the incidence of X. albilineans.
Authors and Affiliations
Aydiloide Bernal Villegas, Pablo Machado Armas, Dunia Núñez Jaramillo, Edel Alejandro Toledo Rodríguez, Rafael Gómez Kosky, Aldo Sergio Noguera, Atilio Pedro Castagnaro
Keywords
Related Articles
- EP ID EP697727
- DOI -
- Views 48
- Downloads 0
Protocol forin vitro propagation of Zingiber officinale Roscoe via organogenesis
Ginger (Zingiber officinale Roscoe) is traditionally propagated by rhizomes and can carrydiseases that decrease production.In vitro propagation allows healthy seed to beobtained, in high volumes and in less time. The obj...
Somatic embryo formation from cotyledons immature in Phaseolus vulgaris cv. CIAP 7247
Common bean, like most of legumes, is recalcitrant to in vitro culture, so the establishment of a regeneration procedure via somatic embryogenesis in this specie has not been possible. Therefore, determining the effect...
Obtention of somatic embryos of Parajubaea cocoides Burret from immature zygotic embryos
Cumbé coconut palm (Parajubaea cocoides Burret) is an ornamental species endemic of Ecuador. It is threatened by environmental and socioeconomic factors. Your sexual propagation by seed, is not effective. Tissue cultur...
Protocol for the artificial inoculation of plants of Musa spp. with Mycosphaerella fijiensis and evaluation of its response using epiphytological variables and components of the resistance
The use of assessment methods of Musa response to natural infection with M. fijiensis in field has allowed differentiation of susceptible, partially resistant and resistant genotypes to black Sigatoka. However, artifici...
Effect of the cytokinins combination on adventitious buds formation in banana cv. ‘Gros Michel’ (Musa AAA)
Cytokinins are used for adventitious buds formation but high concentrations may have negative effects on plant regeneration. The aim of the present investigation was to determine the effect of different combinations of c...