In vitro propagation of Hylocereus purpusii Britton & Rose, a mexican species in danger of extinction
Journal Title: Biotecnología Vegetal - Year 2012, Vol 12, Issue 2
Abstract
The purpose of this work was to evaluate different conditions and culture parameters for the in vitro establishment and multiplication of Hylocereus purpusii. Seeds were used as plant material and a workflow was developed as an alternative for the propagation and recovery of this species in danger of extinction. In the establishment phase, the best result was obtained in the treatment with 1 % NaOCl for 15 minutes achieving a 90% of germinated seeds and a final 77.7% of in vitro established plants. In the multiplication phase when combining 8.88 μM 6-BAP with different concentrations of IAA, a significant increment in the length of the shoots was observed with respect to the control (no addition of IAA). A combination of 8.88 μM 6-BAP with 3.42 μM IAA was selected as the best multiplication condition reaching a multiplication rate of 8.8. In the acclimatization phase, after two months of culture in ex vitro conditions, the survival was higher than 98% and after six months of growth, the plants were transplanted to field.
Authors and Affiliations
Manuel de Feria , Daniel Rojas , Maité Chávez , Mireya Reyna 2, Elisa Quiala , Juan Solís , Florentina Zurita
Keywords
Related Articles
- EP ID EP683712
- DOI -
- Views 135
- Downloads 1
Determination of the adequate concentration of Juglone to differentiate two cultivars of Musa spp.
The toxins production by fungal pathogenic can enhance penetration and colonization of the host tissues and reproduce symptoms of diseases. Juglone is the main and the most toxic metabolite characterized from the culture...
In vitro response of banana plants cv. ‘Grande naine’ (Musa AAA) to water stress induced with polyethylene glycol
Bananas and plantains are grown in tropical and subtropical regions of the world. These plants required high humid conditions; therefore water deficit affects their growth and productivity. Through biotechnology, new d...
Influence of different factors on the in vitro asymbiotic germination of Cattleya labiata seeds
Cattleya labiata is an specie that has been described as ¨Hard Germinate¨ in vitro culture, this investigation was realized to value some factors that can influence on seed germination asymbiotic in vitro in this specie....
Influence of growth regulators and inhibitors on the multiplication of axillary shoots of the hybrid cultivar 'FHIA-21' (AAAB) in Temporary Immersion System
This work was carried out at the Plant Biotechnology Laboratory from the Instituto de Investigaciones en Viandas Tropicales (INIVIT) in order to determine the culture medium for the multiplication of hybrid ‘FHIA–21’ (A...
Production of taxoids in callus and cell suspensions of Taxus chinensis through the use of abiotic elicitors
Until now, several strategies have been developed to improve the production of secondary metabolites using plant cell cultures, manipulating the parameters of the environment, selecting high yielding cell clones and the...