Background: Malaria is one of the important public health problems of African and south east Asian countries including India which contributes to approximately 75% of malaria cases in South East Asia. Some areas of states such as Rajasthan Gujrat Karnataka Jharkhand and Orissa have a very high incidence of malaria with an Annual parasite index more than 5. Behavioral interventions such as public awareness programs rallies and educational camps are found to be very effective in decreasing the incidence of malaria. The biological interventions such as use of Gambusia fish have been found to be effective measures in decreasing the incidence of malaria. Materials and Methods: Following Behavioral and biological interventions the incidence of malaria was analyzed over a period of 7 years from 2009-2016. During this period 600 natural water bodies and 72 hatcheries were made and Gambusia fishes were used to prevent the growth of mosquitoes in water bodies. Information education and communication techniques such as rallies demonstrations and information camps were organized. The purpose of these IEC activities was to make public aware about the personal prevention of mosquito bites. The reduction in cases and mortality due to malaria was analyzed. For Statistical Purposes SSPE 16 software was used. Results: Following Behavioral and Biological Interventions such as allies demonstrations and information camps and use of Gambusia fish as larvicidal there was a significant reduction in the incidence of Malaria in areas covered under Primary health care centers/community health care centers namely Gajner Barsalpur Diyatra Godu and Kolayat RD820 Bajju Akkasar Bikampur and Gadiyala. There was a statistically significant reduction in API in all the regions over a period of 7 years from 2009 to 2016. Conclusion: IEC activities leading to behavioral modification and use of biological agents such as Gambusia fish as larvicidal are found to have a profound impact in reduction in incidence of malaria. Keywords: Malaria Annual Parasite Index Behavior modification Gambusia Fish.