EFFECT OF CENTELLA ASIATICA (LINN) LEAVES ON SELECTIVE MITOCHONDRIAL AND CYTOSOLIC ENZYMES IN STREPTOZOTOCIN INDUCED DIABETIC RATS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objective: The objective of the study is to evaluate the therapeutic effect of Centella asiatica leaves by assaying the activities of selective mitochondrial and cytosolic enzymes in the streptozotocin induced diabetic rats.Methods: The rats were divided into five groups of 6 rats in each group. The methanol extract of Centella asiatica leaves (CALEt) was orally administered at a dose of 300 mg/kg body weight (b. w). The activities of both mitochondrial and cytosolic enzymes viz succinate dehydrogenase (SDH), malate dehydrogenase (MDH), glutamate dehydrogenase (GDH), lactate dehydrogenase (LDH) and glucose 6 phosphate dehydrogenase (G6PDH) were measured in the liver and kidney of the experimental rats.Results: Diabetic rats showed a significant (p<0.001) reduction in the activities of mitochondrial enzymes such as SDH, MDH and GDH. Furthermore, the cytosolic enzyme G6PDH activity was significantly (p<0.001) decreased along with a significant increase in the LDH activity in diabetic rats. The daily oral treatment of CALEt to diabetic rats for 30 d reversed the above changes in a significant (p<0.001) manner.Conclusion: The results obtained in this study indicated that methanol extract of Centella asiatica leaves could restore the altered activities of mitochondrial and cytosolic enzymes, thereby suggesting its position in the energy production. Our data suggest that Centella asiatica leaves should be further explored for its role in the treatment of diabetes mellitus.Â
Authors and Affiliations
Somara Sasikala, Siddamsetty Lakshminarasaiah, Malepati Dhananjaya Naidu
Keywords
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