Effect of delayed vial entry on the recovery and time to positivity of microorganisms from automated blood culture vials in a tertiary care hospital, South India
Journal Title: IP International Journal of Medical Microbiology and Tropical Diseases - Year 2018, Vol 4, Issue 4
Abstract
Recommendations by manufacturers depict that blood culture bottles are to be loaded into the automated machines as soon as possible after the blood collection But it has been noticed that in peripheral setups where round the clock laboratory facility is not available or which are located far from the reference labs or even in the tertiary care centres delay occurs prior to the bottle loading into the instruments In our study we have compared the effect of preincubating the blood culture bottles at various temperatures like 4C room temperature and 37C for various holding times such as 2 6 12 and 24 hours simulating the delay in transport time in BACTEC plus Aerobic and BACTALERT FA plus systems in a tertiary care set up We included five recent clinical isolates of different microorganisms in our study such as Staphylococcus aurous Streptococcus Escherichia coli Pseudomonas aeruginosa and Candida albicans Standard inoculum was prepared for each organism and 3 ml of it was added with 10 ml of citrated human blood and then inoculated into blood culture vial BACTALERT FA plus and BACTEC Plus Organism recovery rate time to detection and rates of falsenegativity from both the instruments were evaluated by using seeded blood culture vial and controls with delayed entry Performance wise analysis showed that the overall isolation rate of the organisms from BACTEC bottles was higher compared to BACTALERT Also BACTEC showed less average TTD compared to BACTALERT for Pseudomonas aeruginosa Streptococcus and candida albicans but for E coli and S aureus BACTALERT showed earlier detection To conclude storage of the inoculated bottles at room temperature gave optimal recovery of the organisms for both BACTEC and BACTALERT systems if delayed entry is inevitableKeyword Delayed vial entry effect on recovery of microorganisms
Authors and Affiliations
Apurba Sankar Sastry, Subhashree Samantaray, Abisha Dr, Suman Susan Prakash, Deepashree R.
Keywords
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- EP ID EP485888
- DOI 10.18231/2581-4761.2018.0054
- Views 49
- Downloads 0
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