Effect of different cupper concentration in culture media on Agave fourcroydes L. in vitro plants
Journal Title: Biotecnología Vegetal - Year 2014, Vol 14, Issue 2
Abstract
A research was realized in order to determine the effect of copper on henequen (Agave fourcroydes L.) var. ‘Sac ki’ in vitro plants. CuSO 4 (25, 50, 75, 100 μM) was added to the culture medium. After 30 days of the in vitro plants established, the following indicators were determined: biomass production, soluble carbohydrates, reducing sugars, soluble proteins, specific activity of catalase and peroxidase enzymes and number of shoots. No phenotypic abnormalities or toxicity symptoms were observed in plants. Biomass showed an increase at the concentration of 50 μM also as the number of shoots at 25 μM. It was proven that the effect on the evaluated indicators was related to the concentration of copper and indicated the tolerance to copper. The results of this investigation settle down the bases for the possible use of this specie in bioremediation of contaminated soils.
Authors and Affiliations
Mohamed Sankoumba Fadiga, Cúc Ðoàn ThË Thu, Yunel Pérez, Gerardo González
Keywords
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