Effect of Different Inactivators on the Efficacy of Egyptian Foot and Mouth Disease SAT2 vaccine
Journal Title: Journal of Animal Science Advances - Year 2013, Vol 3, Issue 8
Abstract
In 2012, Foot and Mouth Disease Virus (FMDV) of serotype SAT2 caused a devastating FMD epidemic in Egypt. In order to control it, a vaccine was produced, based on the Egyptian SAT2 field isolate. As it is often difficult to produce good vaccines from FMDV isolates of the SAT serotypes, a study was carried out to determine the optimal inactivation protocol for the Egyptian SAT2 strain. In comparison to the usual FMDV inactivation protocol with binary ethyleneimine (BEI) alone, an alternative protocol was employed which used formalin as an additional inactivating agent and possible antigen stabilizer in combination with BEI. Inactivation kinetics was recorded for both protocols and the preservation of the antigens were determined by complement fixation test (CFT). From both antigens trial vaccines were formulated with Montanide ISA 206 and tested in guinea pigs. While inactivation with 0.1 M BEI alone resulted in an inactivation rate of 0.53 log10 TCID50/hour, BEI combined with formalin (BEI-FA) resulted in a significantly higher inactivation rate of 2 log10 TCID50/hour. While BEI inactivation of the antigen led to a drop in CFT titer from 1/32 to 1/16, no such decline was found with the BEI-FA inactivation protocol. The BEI-FA protocol also resulted in a trial vaccine with higher immunogenicity in guinea pigs. For 28 days post vaccination (dpv) sera, SNT and ELISA titers of 1.8 and 2.05, respectively, were recorded for the BEI inactivated antigen while for BEI-FA inactivated antigen, SNT and ELISA titers of 2.1 and 2.3, respectively, were recorded. Finally it was clear that, the use of BEI-FA as an inactivator for FMDV (SAT2) reduces the time of inactivation and provides good and safe antigen content consequently higher post vaccinal antibody titer.)
Authors and Affiliations
E. M. Soliman , S. E. Mahdy , W. G. Mossad , A. I. Hassanin, E. I. El-Sayed
Keywords
Related Articles
- EP ID EP146502
- DOI -
- Views 119
- Downloads 0
The Growth Performance, Carcass and Haematological Characteristics of Broiler Chickens Fed Alkaline-Treated Tiger Nut (Cyperus Esculentus) Residue Meal
A fifty six (56) day repeated feeding trial was conducted to investigate the nutritive value of alkaline-treated tiger nut residue meal (ATTNRM) and raw tiger nut residue meal (RTNRM) using 240 unsexed day-old broiler ch...
In Situ Digestion of Forages and Grains in Alpacas Fed Alfalfa and Grass Hay
In situ disappearance of alfalfa hay, grass hay, corn, corn/oats/barley and oat DM and NDF in alpacas fed alfalfa hay (AHF) or grass hay (GHF) was determined in adult male alpacas. The alpacas were then divided into two...
Responses of Growing Rats and Pigs to Diets Containing Varying Levels of Dried Brewers’ Spent Grains Supplemented with Bergazym-an Exogenous Enzyme Complex.
Grower pigs (n=32) with mean initial weight of about 15.38kg were randomly allocated to four dietary treatments based on sex and live weight in a Randomized Complete Block Design. Each treatment had four (4) replicates o...
Study on Ovine Fasciollosis in Wegdi District, North East Ethiopia
A Cross-sectional study was conducted in selected Kebele’s of Wegdi Wereda of South Wollo zone from October 2011 up to December 2011with the objective of estimating the prevalence and related risk factors of fasciolosis...
Milk Quality of Dairy Cattle Bred in Ceará, Northeast of Brazil
The demand for high quality dairy products is growing, milk should be produced under both sanitary and technological standards, to ensure maintenance of its nutritional characteristics and meet the required hygienic aspe...