Effect of Electron Beam Irradiation on Viability of Sarcocystis spp. in Beef
Journal Title: Journal of Food Quality and Hazards Control - Year 2020, Vol 7, Issue 4
Abstract
Background: Sarcocystosis is one of the most distributed parasitic diseases over the world, caused by Sarcocystis spp. In this study, we assessed the effect of electron beam irradiation on the viability of Sarcocystis spp. in beef. Methods: Experimental beef groups were irradiated by four different electron beam doses of 1, 2, 3, and 4 kGy, at intervals of 0 and 24 h after irradiation, then the samples were transferred inside a sterile microtube containing RNAlater solution and stored at -20 °C till next steps. RNA extractions and cDNA synthesis were done using the related kit in order to detect the presence of the 18S ribosomal RNA region. Relative quantification was carried out using SYBR Green Real time Polymerase Chain Reaction. The statistical analysis was done using SPSS 16.0 by Tukey’s and Kruskal-Wallis tests. Results: Irradiation at zero time was not effective on viability of Sarcocystis, but at 24 h, irradiation doses of 3 (p=0.003) and 4 kGy (p=0.008) caused a significant reduction in Sarcocystis viability. Irradiation doses of 1 and 2 kGy had no significant (p>0.05) effect on Sarcocystis viability reduction. Also, no significant differences (p>0.05) were observed between irradiation doses of 3 and 4 kGy. Conclusion: Electron beam radiation at dose of 3 kGy was effective as the optimal dose for the elimination of Sarcocystis spp. in beef.
Authors and Affiliations
G. Eslami, S. Peletto, M. Vakili, S. Kargar*
Keywords
Related Articles
- EP ID EP690945
- DOI 10.18502/jfqhc.7.4.4847
- Views 126
- Downloads 0
Oxidative and Frying Stabilities of Monodora myristica (Gaertn.) Dunal Seed Oil of Nigerian Origin
Background: The demand for vegetable oils is on the increase. Deep frying is the commonest method by which vegetable oils are consumed. The aim of this study was to extract oil from an underutilized oil seed and compare...
Antibiotic Resistance and Biofilm Production in Catalase-Positive Gram-Positive Cocci Isolated from Brazilian Pasteurized Milk
Background: Milk is a reservoir for several groups of microorganisms, which may pose health risks. The aim of this work was to assess the antibiotic resistance and biofilm production in catalase-positive Gram-positive co...
The Occurrence of Serotypes and Virulence Genes of Listeria monocytogenes in Various Food Products
Background: Given that controlling Listeria contamination is very important in food chain system, the knowledge of their prevalence in food is very important. Therefore, this study aims to examine the prevalence of impor...
Direct Molecular Detection and Phylogenetic Tree Analysis of Gastrointestinal Protozoan Parasites (Giardia lamblia, Entamoeba histolytica, Cryptosporidium parvum) from Diarrhea Infection in Kut City of Iraq: A Short Communication
Background: The intestinal tract of human can be infected by protozoan parasites. In this short communication, the stool samples were collected from patients with diarrhea referred to Kut hospital, Iraq, and then the par...
Sea Grapes Paper Towel Enriched with Activated Carbon: A Practical Innovation to Reduce Acrylamide in Fried Food
Background: One of the possible carcinogenic substances found in foods is Acrylamide (AA). This study aims to combine and innovate sea grapes (Caulerpa racemosa) extract with activated carbon into paper towels that can a...