Effect of foliar injection of juglone on Superoxide dismutases antioxidant system in two cultivars of Musa spp.
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 2
Abstract
Some diseases in higher plants cause formation of reactive oxygen species (ROS) which acts like mediator on tolerance to oxidative stress. Several phytotoxins produced by plant pathogen fungus induce ROS by different mechanisms which damage plant cell tissues. This paper was focused to determine juglone impact (Psedocercospora fijiensis toxin) on Superoxide dismutases antioxidant system in Fougamou (ABB) and Grande naine (AAA) by native Poliacrylamide gel electrophoresis (PAGE) 10%. Samples of proteins were collected at 2, 4, 6, 8 and 48h after juglone injection on both Musa cultivar and 65 μl of them (120 μg of total protein) were applied to gels. Gels were incubated in staining solution (25 mg.ml -1 de Nitrobluetetrazolium (NTB) and 0.1 mg.ml -1 de riboflavin) and were exposured to fluorescent light. In both cultivars it was observed changes in expression patterns of superoxide dismutases between injected and not injected plants. On Grande naine (AAA) 4 h after injection an isoform of SOD disappeared respect to control. Nevertheless on Fougamou (ABB), 2 h after injection it was activated a new isoform which was observed until 48 h. In the present work it was observed a correspondence between protein patterns expression of SOD isoforms and tolerance to oxidative stress caused by the effect of juglone on Musa spp.
Authors and Affiliations
Michel Leiva Mora, Jean Pierre Bussogoro, Philippe Lepoivre
Keywords
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