Effect of Follicular Fluid on in Vitro Maturation, Fertilization and Development of Goat Embryos using Fresh Semen
Journal Title: International Journal of Agriculture Innovations and Research - Year 2018, Vol 6, Issue 6
Abstract
The study was undertaken to find out the beneficial effect of goat follicular fluid (FF) on in vitro maturation, fertilization using fresh semen and to support the subsequent goat embryo development. Cumulus oocytes complexes (COCs) were collected from goat ovaries by aspiration method and matured for 24 hours in TCM-199 basic medium supplemented with different levels of FF (5%, 10% and 15%). The percentages of COCs reached to Metaphase-II stages with 0%, 5%, 10% and 15% of FF supplementation were 43.33 ± 3.33, 51.67 ± 0.83, 66.66 ± 0.00 and 67.79 ± 1.92% respectively. The normal fertilization (formation of two pronuclei) was recorded as 27.47 ± 0.55, 31.69 ± 1.09, 36.54 ± 1.73 and 38.69 ± 2.95%, respectively. After fertilization the rate of development to compact morula was found as 7.95 ± 1.35, 10.17 ± 0.17, 13.34 ± 3.33 and 12.63 ± 1.33%, respectively. These results further indicate that the maturation, fertilization and subsequent development rate could be significantly increased (p0.01) by supplementing 5% level of FF than that of control. This trend maintained up to 10% of FF, but no further improvement was found by increasing the level of FF up to 15%. Thus, this study suggests that follicular fluid supplementation at 10% level could be beneficial for maturation and culture of goat oocytes and embryos.
Authors and Affiliations
L. Asad, et al.
Keywords
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