Effect of Follicular Fluid on in Vitro Maturation, Fertilization and Development of Goat Embryos using Fresh Semen
Journal Title: International Journal of Agriculture Innovations and Research - Year 2018, Vol 6, Issue 6
Abstract
The study was undertaken to find out the beneficial effect of goat follicular fluid (FF) on in vitro maturation, fertilization using fresh semen and to support the subsequent goat embryo development. Cumulus oocytes complexes (COCs) were collected from goat ovaries by aspiration method and matured for 24 hours in TCM-199 basic medium supplemented with different levels of FF (5%, 10% and 15%). The percentages of COCs reached to Metaphase-II stages with 0%, 5%, 10% and 15% of FF supplementation were 43.33 ± 3.33, 51.67 ± 0.83, 66.66 ± 0.00 and 67.79 ± 1.92% respectively. The normal fertilization (formation of two pronuclei) was recorded as 27.47 ± 0.55, 31.69 ± 1.09, 36.54 ± 1.73 and 38.69 ± 2.95%, respectively. After fertilization the rate of development to compact morula was found as 7.95 ± 1.35, 10.17 ± 0.17, 13.34 ± 3.33 and 12.63 ± 1.33%, respectively. These results further indicate that the maturation, fertilization and subsequent development rate could be significantly increased (p0.01) by supplementing 5% level of FF than that of control. This trend maintained up to 10% of FF, but no further improvement was found by increasing the level of FF up to 15%. Thus, this study suggests that follicular fluid supplementation at 10% level could be beneficial for maturation and culture of goat oocytes and embryos.
Authors and Affiliations
L. Asad, et al.
Keywords
Related Articles
- EP ID EP501525
- DOI -
- Views 87
- Downloads 0
A Screen House Experiment to Evaluate the Biostimulation Potentials of Cow Dung on a Crude Oil Polluted Soil
This screen house study was conducted to investigate the effect of the application of cow dung to crude oil polluted soils. Four rates of cow dung (0, 1, 2 and 3g) and four rates (0, 5, 10 and 15ml) of crude oil per 10kg...
Technological Gap Analyses of Mena and Southern African Countries’ Agricultural Productivity
The technical gap analysis of countries’ agricultural productivity was analyzed and the discussions of the study were divided into two regions of Middle East and North African (MENA), and Southern Africa (SA) Countries b...
Effect of Irrigation Schedule and Nitrogen Management on Productivity, Profitability of Summer Pearl Millet Grown Under Clay Soils of South Gujarat
A field experiment was carried out at Soil and Water Management Research Farm, Navsari, on clayey soils during summer season of 2016 to study the “Effect of irrigation schedule and nitrogen management on productivity, pr...
Arthropoda Abbreviation of Natural Enemy (Predator) on the Soybean Plantation in the Sub of Optimal Sub District Jayapura Papua
Production of soybean in Papua Province in 2017 amounted to 3522.53 tons with 2761 ha harvested area with low productivity which is average 0.77 -1.3 tons / ha. Jayapura district is one of the soybean production centers...
Evaluation of Markov Chain Model for Forecasting Precipitation of Uttarakhand Districts
This paper intends to evaluate the Markov Chain model for forecasting rainfall of Uttarakhand state for each district separately. District wise interpolated daily rainfall data for the period 1971 – 2011 was collected fr...