Effect of high dietary copper on the expression of hypothalamic appetite regulators in weanling pigs
Journal Title: Journal of Animal and Feed Sciences - Year 2011, Vol 20, Issue 1
Abstract
The current study evaluated appetite-related gene expression of neuropeptide Y (NPY), orexin, type 2 orexin receptor (OrexinR2), proopiomelanocortin (POMC), melanocortin-4 receptor (MC4R), AMP-activated protein kinase α2 subunit (AMPKα2) and long-form leptin receptor (LeptinRb) in hypothalamus in response to copper (Cu) supplementation. One hundred crossbred pigs were assigned to four groups of 25 pigs, each comprising five replicates of 5 animals. Groups were then randomly assigned to treatments consisted of 1. control (10 mg/kg CuSO4 ), 2. 100 mg/kg CuSO4 , 3. 175 mg/kg CuSO4 , 4. 250 mg/kg CuSO4 . On d 21 of the experiment 5 pigs from each group were slaughtered and the hypothalami were collected for determination of appetite-regulating genes mRNA expression level. The results showed that average daily feed intake and average daily gain were higher (P<0.05) in 250 and 175, 250 mg/kg Cu supplemented groups, respectively, than in the 10 mg/kg group. Feed:gain ratio was lower in pigs fed the diets with 250 mg/kg Cu (P<0.05) than in the 10 mg/kg group. Furthermore, the abundances of NPY mRNA in hypothalamus were higher in 250 mg/kg Cu supplemented groups (P<0.05), whereas the abundances of POMC and LeptinRb mRNA were significantly reduced (P<0.05) in 170, 250 mg/kg and 100, 175, 250 mg/kg Cu supplemented groups, respectively. No difference was found for AMPKα2, MC4R, orexin, OrexinR2 mRNA expression (P>0.05). These data suggest that down-regulation of LeptinRb mRNA expression might contribute to the stimulation of feeding of high dietary Cu supplementation via regulation of NPY and POMC mRNA expression.
Authors and Affiliations
D. Zhu, B. Yu, C. Ju, S. Mei
Keywords
Related Articles
- EP ID EP74707
- DOI -
- Views 100
- Downloads 0
Dietary conjugated linoleic acid isomers and selenite or selenized yeast affect fatty acid concentration in the kidneys of rats
The influence of diets enriched in a mixture of conjugated linoleic acid (CLA) isomers (CLAmix) and/or selenium as Na2 SeO3 (SeIV) or selenized yeast (SeY) on feed intake, kidneys’ weight and the concentrations of fatty...
Possibility of utilization of two Aegilops sp. to enhance the nutritive value of triticale
Study was undertaken to determine the possibility of improving the nutritive value of triticale by crossing it with two species of Aegilops. Materials for this study comprised of 5 triticale recombinants with Aegilops cr...
The effect of dietary yellow lupine (L. luteus cv. Baryt) on growth performance, carcass characteristics, meat quality and selected serum parameters of turkeys
This study evaluated the effects of dietary replacement of soyabean meal (SBM) with yellow lupine seed meal (YLM) in turkeys. A total of 1120 one-day-old Hybrid Converter female turkeys were fed diets containing 0%, 8%,...
The nutritive value of maize cultivars for broiler chickens
The nutritive value of maize cultivars for broiler chickens
Practical validation of efficacy of the standardized ileal digestible amino acid values in diet formulation for broiler chickens
The aim of experiment was to determine the potential for the improvements in growth performance and carcass traits, and reduction of N excreta output in final rearing period of broiler chickens by formulating diets on st...